Merck
All Photos(1)

SRP6170

Sigma-Aldrich

IL-2 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):
T-cell growth factor (TCGF), Lymphokine, N/A, Aldesleukin
CAS Number:
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized

potency

0.2-2 ng/mL

mol wt

15 kDa (monomer, glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... IL2(3558)

General description

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.
The IL-2 gene is mapped to human chromosome 4q27.

Application

IL-2 (interleukin 2) human has been used for the stimulation of human primary naive T lymphocytes to study the effect of microRNA-146a on IL-2 activation-induced cell death.

Biochem/physiol Actions

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL-2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.

Physical form

Lyophilized from a PBS solution.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

An emerging player in the adaptive immune response: microRNA-146a is a modulator of IL-2 expression and activation-induced cell death in T lymphocytes.
Curtale G
Blood, 115, 265-273 (2010)
Family based association analysis of the IL2 and IL15 genes in allergic disorders.
Christensen U, et al.
European Journal of Human Genetics, 14(2), 227-227 (2006)
Associations of IL-2 and IL-4 Expression and Polymorphisms With the Risks of Mycoplasma pneumoniae Infection and Asthma in Children.
Archivos de bronconeumologia, 51, 571-578 (2015)
Prostaglandin E2 (PGE2)-dependent suppression of interleukin alpha (IL-2) production in patients with major trauma.
Faist E
The Journal of Trauma, 27, 837-848 (1987)
Microarray analysis of potential genes in the pathogenesis of recurrent oral ulcer.
Han J
International Journal of Clinical and Experimental Pathology, 8, 12419-12427 (2015)

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