Merck
All Photos(1)

Documents

STR1

Sigma-Aldrich

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

All Photos(1)
Synonym(s):
Reverse Transcriptase cDNA synthesis kit, eAMV-RT
NACRES:
NA.55

Quality Level

200

usage

sufficient for 50 reactions
 kit sufficient for 50 reactions

feature

dNTPs included
hotstart: no

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

dry ice

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A446411483188001GE27-9261-01
Sigma-Aldrich

Sigma-Aldrich

STR1

Enhanced Avian First Strand Synthesis Kit

Enhanced Avian Reverse Transcriptase [eAMV™ RT] For reverse transcription at higher temperatures & rare mRNAs

Sigma-Aldrich

A4464

Enhanced Avian Reverse Transcriptase [eAMV™ RT]

First Strand cDNA Synthesis Kit for RT-PCR (AMV) sufficient for 30 reactions (including 5 control reactions), kit of 1 (10 components), suitable for RT-PCR, hotstart: no, dNTPs included

Roche

11483188001

First Strand cDNA Synthesis Kit for RT-PCR (AMV)

First-strand cDNA Synthesis Kit Cytiva 27-9261-01

GE27-9261-01

First-strand cDNA Synthesis Kit

technique(s)

RT-PCR: suitable

technique(s)

RT-PCR: suitable

technique(s)

RT-PCR: suitable

technique(s)

-

input

purified RNA

input

purified RNA

input

purified RNA

input

-

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

General description

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

Application

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).

Unit Definition

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Kit Components Only

Product No.
Description
  • Enhanced Avian Reverse Transcriptase 1000 U

Kit Components Also Available Separately

Product No.
Description
SDS
  • Deoxynucleotide mix 50 μL
  • O4387Anchored oligo (dT)23 100 μLSDS
  • R7647Random nonamers 100 μLSDS
  • O4387Ribonuclease inhibitor 50 μLSDS
  • W1754PCR grade water 1.5 mLSDS

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Oligo(dT)23, Anchored 70 μM in H2O

Sigma-Aldrich

O4387

Oligo(dT)23, Anchored

KAPA PROBE FAST One-Step Universal

Roche

KK4752

KAPA PROBE FAST One-Step

Sigma-Aldrich

Sigma-Aldrich

R7647

Random Nonamers

Transcriptor Universal cDNA Master sufficient for 100 reactions, suitable for cDNA synthesis, suitable for RT-PCR, suitable for RT-qPCR

Roche

05893151001

Transcriptor Universal cDNA Master

JianFei Wang et al.
Journal of leukocyte biology, 82(6), 1554-1563 (2007-11-28)
Wound healing is a complex process involving the integrated actions of numerous cell types, soluble mediators, and ECM. Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that
Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
Heat shock proteins expression during thermal risk exposure in the temperate xerothermic ant Formica cinerea
Slipinski P, et al.
Sociobiology, 62(3), 457-459 (2015)
Julio Carrion et al.
Journal of immunology (Baltimore, Md. : 1950), 189(6), 3178-3187 (2012-08-15)
The low-grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. In this study, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic
View All Related Papers

Articles

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

Protocols

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

Related Content

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service