Merck
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T0377

Sigma-Aldrich

Tricine

≥99% (titration)

Synonym(s):
N-[Tris(hydroxymethyl)methyl]glycine
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
Beilstein:
1937804
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99% (titration)

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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This Item
T9784T17917RES3077T-A7
Tricine ≥99% (titration)

Sigma-Aldrich

T0377

Tricine

Tricine BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)

Sigma-Aldrich

T9784

Tricine

Tricine ≥99% (titration), reagent grade, crystalline

Sigma-Aldrich

T17917

Tricine

Tricine

SAFC

RES3077T-A7

Tricine

assay

≥99% (titration)

assay

≥99% (titration)

assay

≥99% (titration)

assay

-

form

crystalline powder

form

powder

form

crystalline

form

powder

mp

187 °C

mp

-

mp

-

mp

-

solubility

water: 0.25 g/mL, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

400

General description

Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.

Application

Tricine has been used:
  • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures
  • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants
  • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay

Buffer component for separation of low molecular weight peptides.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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