Merck
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V5265

Sigma-Aldrich

Crystal violet solution

1%, aqueous solution

Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
Beilstein:
3580948
MDL number:
PubChem Substance ID:

Quality Level

form

aqueous solution

concentration

1%

Amax

0.36-0.44 at 589-594 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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General description

Crystal violet, also known as gentian violet, is a protein dye and is basic in nature. It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

Application

Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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