WGA1
GenomePlex® Whole Genome Amplification (WGA) Kit
Kit for whole genome amplification from a variety of DNA sources including FFPE tissue
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General description
WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.
Application
Features and Benefits
- Higher yield from minimal template: Amplification of nanogram amounts (10ng) of genomic DNA to microgram yields (5-10 μg) in less than about three hours
- Nanograms of samples can be preserved at –20 °C for future use
- Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, and formalin-fixed paraffin-embedded tissue (FFPE)
- Compatible with a wide variety of downstream applications such as TaqMan® and BeadArray assays
- Universal primers
- Whole-genome representation with no detectable bias
- Increased accuracy in amplification produces no amplicon in the negative control reactions
Other Notes
Legal Information
Kit Components Also Available Separately
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
recommended
related product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
Protocols
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Related Content
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
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