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ZMS1075

Sigma-Aldrich

Anti-SARS-CoV-1/2 NP Antibody, clone 1C7C7 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
Nucleocapsid protein, Protein N., NC

Quality Level

biological source

mouse (recombinant)

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C7C7, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 45.63 kDa (SARS-CoV-2)
calculated mol wt 46.03 kDa (SARS-CoV)
observed mol wt ~46 kDa

species reactivity

SARS virus

packaging

antibody small pack of 25 μL

greener alternative product characteristics

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

affinity binding assay: suitable
flow cytometry: 1 μg/mL
immunocytochemistry: suitable
western blot: 1 μg/mL

isotype

IgG2aκ

conjugate

unconjugated

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

SARS coronavirus ... N(43740575)
SARS virus ... N(1489678)

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1C7C7 is a ZooMAb mouse recombinant antibody that specifically detects SARS-CoV-1/2 NP.

Immunogen

His-tagged full-length recombinant Nucleoprotein from SARS-CoV virus.

Application

Flow Cytometry Analysis: 1 mg /ml from a representative lot detected SARS-CoV-1/2 NP in Transfected 293expi cells (Courtesy of Dr Thomas Moran, Center for Therapeutic Antibody Development).

Immunocytochemistry Analysis: 1 μg/mL from a representative lot detected SARS-CoV-1/2 NP in Vero E6 cells infected with SARs-CoV-2 (Courtesy of Dr Ana Fernandez-Sesma Laboratory, Icahn School of Medicine at Mount Sinai (ISMMS), New York).

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SARS-CoV-1/2 NP in lysate from A549 cells infected with SARS-CoV-2 (Courtesy of Jeff Johnson Laboratory, Icahn School of Medicine at Mount Sinai (ISMMS).

Affinity Binding Assay: This antibody bound recombinant nucleoprotein from SARS-CoV-1/2 with a KD of 4.4 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SARS-CoV-1/2 NP, clone 1C7C7 ZooMAb, Cat. No. ZMS1075, is a Mouse monoclonal antibody that detects nucleoprotein from SARS-CoV-1/2 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Target description

Nucleoprotein (UniProt: P59595/P0DTC9; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 1489678) in Human SARS coronavirus. The SARS-CoV-2 (COVID-19 causing virus) is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of SARS-CoV and SARS-CoV-2 is a homooligomeric protein that packages the positive strand viral genome RNA into helical ribonucleocapsid and plays a role in virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. The RNA binding region of SARS-CoV and SARS-CoV-2 is localized to amino acids 42-187 and 41-186, respectively. Lack of cysteine is one of the common features in nucleoprotein from different coronaviruses. The complete N protein of SARS-CoV and COV-2 is a highly basic with positive net charges and high pI (10.11). Its N-terminal region is more basic with positive charge, while the C-terminal region is acidic with negative charge. The middle region of nucleoprotein displays relatively higher hydrophobicity and the two termini display greater hydrophilicity. Nucleoprotein can undergo phosphorylation at multiple serines by GSK-3, CKII, Akt, CDKs, and Polo-like kinases. Some of these phosphorylation events are essential for RNA binding, oligomerization and localization to nucleoli. Phosphorylation is also required for recruitment of host RNA helicase DDX1 (DEAD-Box Helicase 1) that facilitates template readthrough and enables longer subgenomic mRNA synthesis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing PBS, 5% Trehalose without preservatives

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Jingqiang Wang et al.
Genomics, proteomics & bioinformatics, 1(2), 145-154 (2005-01-01)
The Coronaviridae family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it...
Jun-Gyu Park et al.
Journal of virological methods, 287, 113995-113995 (2020-10-18)
Towards the end of 2019, a novel coronavirus (CoV) named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), genetically similar to severe acute respiratory syndrome coronavirus (SARS-CoV), emerged in Wuhan, Hubei province of China, and has been responsible for coronavirus disease 2019...
A serological assay to detect SARS-CoV-2 seroconversion in humans.
Amanat, F., et al.
Nature Medicine, 26, 1033-1036 (2020)
Fatai S Oladunni et al.
Journal of virological methods, 290, 114084-114084 (2021-01-30)
The use of monoclonal neutralizing antibodies (mNAbs) is being actively pursued as a viable intervention for the treatment of Severe Acute Respiratory Syndrome CoV-2 (SARS-CoV-2) infection and associated coronavirus disease 2019 (COVID-19). While highly potent mNAbs have great therapeutic potential...
Milan Surjit et al.
Journal of virology, 79(17), 11476-11486 (2005-08-17)
The severe acute respiratory syndrome coronavirus(SARS-CoV) nucleocapsid (N) protein is one of the four structural proteins of the virus and is predicted to be a 46-kDa phosphoprotein. Our in silico analysis predicted N to be heavily phosphorylated at multiple residues....

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