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ZRB1062

Sigma-Aldrich

Anti-ZFAND1 Antibody, clone 4K16 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Quality Level

biological source

rabbit

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

4K16, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 30.79 kDa
observed mol wt ~30 kDa

purified by

using Protein A

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

ELISA: suitable
affinity binding assay: suitable
western blot: suitable

isotype

IgG

epitope sequence

Unknown

conjugate

unconjugated

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4K16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Zinc finger AN1-type-containing protein 1 (ZFAND1).

Immunogen

His-tagged full-length recombinant human Zinc finger AN1-type-containing protein 1 (ZFAND1).

Application

Anti-ZFAND1, clone 4K16 ZooMAb®, Cat. No. ZRB1062, is a recombinant Rabbit monoclonal antibody that detects Zinc finger AN1-type-containing protein 1 (ZFAND1). (ZFAND1) and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZFAND1 in HeLa cell nuclear extract.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZFAND1 in NIH3T3 cell nuclear extract.

ELISA Analysis: 300 μg/mL of this antibody detected ZFAND1 in an ELISA.

Affinity Binding Assay: A representative lot of this antibody bound ZFAND1 with a KD of 6.4 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

AN1-type zinc finger protein 1 (UniProt: Q8TCF1; also known as Zinc finger AN1-type-containing protein 1, ZFAND1) is encoded by the ZFAND1 gene (Gene ID: 79752) in human. ZFAND1 is a 268 amino acid protein with two ANI-type zinc fingers (aa 7-58 and 61-110) and a UBL domain. It plays a role in the regulation of cytoplasmic stress granules (SGs) turnover. It contains six conserved cysteines, two histidines, and has a zinc-bound /β fold. There are four isoforms of ZFAND1 have been described that are produced by alternative splicing. ZFAND1 is reported to interact with two key factors of protein degradation, the 26S proteasome and the ubiquitin-selective segregase p97, via its zinc finger and UBL domains, and recruits them to arsenite-induced stress granules. In the absence of ZFAND1, stress granules lack the 26S proteasome and p97 and accumulate defective ribosomal products. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Turakhiya, A., et al. (2018). Mol. Cell. 70(5); 906-919).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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