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ZRB1389

Sigma-Aldrich

Anti-ITGA3 Antibody, clone 1D6 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Quality Level

biological source

rabbit

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D6, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 116.61 kDa
observed mol wt ~160 kDa

purified by

using Protein A

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

conjugate

unconjugated

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D6 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Integrin a-3 (ITGA3). It targets an epitope within 29 amino acids from the C-terminal cytoplasmic domain. It detects the a-chain and the full-length protein.

Immunogen

KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal, cytoplasmic domain of human Integrin a-3

Application

Anti-ITGA3, clone 1D6 ZooMAb®, Cat. No. ZRB1389, is a recombinant Rabbit monoclonal antibody that detects ITGA3 (CD49c) and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ITGA3 in U2OS cell lysate.

Tested applications


Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ITGA3 in A431 and C2C12 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ITGA3 in A431 cells.

Affinity Binding Assay: A representative lot of this antibody bound ITGA3 with a KD of 6.8 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ITGA3 in human kidney and human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Integrin alpha-3 (UniProt: P26006; also known as CD49 antigen-like family member C, FRP-2, Galactoprotein B3, GAPB3, VLA-3 subunit alpha, CD49c) is encoded by the ITGA3 (also known as MSK18) gene (Gene ID: 3675) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b -subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrin a -3 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 33-991), a transmembrane domain (aa 992-1014), and a cytoplasmic domain (aa 1015-1051). It has two chains- a heavy chain (aa 33-872) and a light chain (aa 876-1051). Integrin a3 dimerizes with integrin b1 to form a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin, and chondroitin sulfate proteoglycan 4 (CSPG4). It provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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