SP®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

SP-2560, 100M.20UM.25MM

Quality Level


fused silica


AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5


≤25-250 °C temperature (isothermal or programmed)

Beta value



0.20 μm


gas chromatography (GC): suitable

L × I.D.

100 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

Featured Industry

Food and Beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Certificate of Origin

Fatty acid composition of human erythrocyte membranes by capillary gas chromatography-mass spectrometry.
Alexander LR
Journal of Chromatography. B, Biomedical Applications, 342, 1-12 (1985)
Adriana Campione et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-05)
The hazelnut skin is waste biomass rich in bioactive compounds that may affect lipid rumen metabolism, ruminant performance, and products' quality. Therefore, we investigated the effect of dietary hazelnut skin on milk production and composition and on rumen fatty acids...
Evaluation of the CP-Sil 88 and SP-2560 GC columns used in the recently approved AOCS official method Ce 1h-05: Determination of cis-, trans-, saturated, monounsaturated, and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC method.
Ratnayake WMN
Journal of the American Oil Chemists' Society, 83 (6), 475-488 (2006)
B Schettino et al.
Journal of dairy science, 100(8), 6256-6265 (2017-06-12)
Chia seed (Salvia hispanica L.) is the greatest known plant source of n-3 α-linolenic acid. The present study evaluated the effects of 3 inclusion levels of chia seed [zero (control); low, 2.7% (CLow); and high, 5.5% (CHigh)] in diets of...
Antonio Natalello et al.
Journal of agricultural and food chemistry, 67(16), 4472-4482 (2019-04-02)
This work investigated the effects of feeding whole pomegranate byproduct (WPB) to lambs on ruminal, liver, and intramuscular fatty acids (FA). Seventeen lambs, divided into two groups, were fed for 36 days with a cereal-based concentrate diet (CON) or with...
A protocol for GC Analysis of a 37-Component FAME Mix on SP™-2560.
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Separation of C8:0; C10:0; C11:0 (I.S.); C12:0; C13:0 (I.S.); C14:0; C14:1n5; C15:0; C16:0; C16:1n7; C17:0; C17:1n7; C18:0; C18:1 trans; C18:1 cis; C18:2n6; C18:2n7; C18:3n6 ; C18:3n3; C20:0; C20:1n9; C20:2n6; C20:3n3; C20:4n6; C22:0; C22:1n9; C22:6n3 (DHA); C24:0
Read More
GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp
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Technical article on GC Analysis of cis/trans C18:1, C18:2, and C18:3 FAME Isomers on SP™-2560
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