3D Cell Culture Techniques

Cells naturally grow and differentiate in 3D environments. Improved 3D cell culture models can more accurately replicate the native tissue environment to provide meaningful scientific conclusions. We offer a broad portfolio of solutions for 3D cell culture including products for supporting spheroid growth, scaffolds, hydrogels, organoids, ULA plates, and other cultureware.

Hydrogels
Cells embedded in 3D hydrogels can polarize, differentiate and have functional attributes that more closely resemble physiological tissues. Choose naturally derived hydrogels or reconstitute a hydrogel from synthetic materials to develop predictive cellular models.
Scaffolds for 3D Cell Culture
Cells grown on structural scaffolds engineered to mimic the ECM can be powerful, predictive models of physiological processes. Our wide range of advanced 3D cell culture scaffolds includes nanofiber, collagen, polystyrene, and polycaprolactone materials.

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Organoids are in vitro models that closely replicate the complex physiology of native tissues while also being accessible, reproducible, and scalable like traditional culture methods. Choose colon organoids, lung organoid culture systems, and patient-derived organoids from our biobank.
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SynVivo SynTumour 3D cancer model chip, microvascular network configuration, 8 um pillar
case of 3 ea

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
liquid, BioReagent, suitable for cell culture

3dGRO® R-Spondin-1 Conditioned Media Supplement
Alternative stem cell media supplement to purified recombinant R-Spondin-1 protein for organoid cultures.

MaxGel™ ECM
mixture, liquid

3dGRO® Human Colon Organoid Expansion Medium
Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

3dGRO® Human iPSC Derived Colon Organoids

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture

Bovine Collagen Type I
from bovine skin, liquid, 5 mg/mL, suitable for cell culture, used for 3D Hydrogel formation, PureCol™ EZ Gel

DMEM/F-12 PLUS Basal Medium
Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.

R-Spondin1 expressing 293T Cell Line
Human

CellCrown™ inserts
96 well plate inserts, 8-well strips, sterile

CellCrown™ inserts
48 well plate inserts, 6-well strips, sterile

3dGRO® Organoid Freeze Medium
Optimized cryopreservation media for multiple organoids cell types.

3dGRO® Organoid Dissociation Reagent
Chemically defined enzyme-free organoid dissociation solution.

TrueGel3D Hydrogel Kits
SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

CellCrown™ inserts
24 well plate inserts, sterile

Nanofiber multiwell plate, with aligned nanofibers
size 96 wells

Nanofiber multiwell plate, with aligned nanofibers
size 24 wells

Nanofiber chamber slides
8 chamber slide, aligned nanofibers

TrueGel3D Hydrogel Kits
CD cell-degradable crosslinker and RGD peptide
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