Merck

Whole Genome Amplification

Complete genome amplification kits

The availability of sufficient quantities of genomic DNA is crucial for numerous analyses used in the study of human and animal disorders. In many cases, the DNA source is limited, or the available DNA is damaged or degraded, preventing the researcher from performing crucial analyses. Our Whole Genome Amplification (WGA) product line provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out. We continue to expand our WGA product line to offer you a range of solutions for whole genome amplification from a variety of starting material, including buccal swabs, fresh tissue samples, frozen tissue samples, blood cards, peripheral blood and formalin-fixed, paraffin-embedded (FFPE) tissues.  The WGA technology allows immortalization of a limited sample providing researchers with the capability to perform all desired analyses on one DNA sample. 



Related Resources

  • Saliva DNA Extraction & WGA Amplification Protocol

    Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.

  • Whole Genome Amplification from Serum or Plasma Protocol

    Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

  • Animal Tissue DNA-Extraction & WGA Amplification Protocol

    GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

  • Plant DNA Extraction & WGA Amplification Protocol

    GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

  • Blood Card - Extraction & Amplification WGA Protocol

    Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol

  • Extraction & Amplification of Whole Blood Using WGA-Protocol

    Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

  • Buccal DNA Extraction & WGA Amplification Protocol

    This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.

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Whole Genome Amplification (WGA) Kits

Genomic testing and characterization have become an important tool for understanding biological systems. Often, such analysis is hampered by the number of samples to be examined and the availability of sufficient quantities of genomic DNA. This is particularly a problem for rare and archived sources of DNA. The GenomePlex® Whole Genome Amplification (WGA) kits have been developed for use as a high-throughput system for the rapid and highly representative, amplification of genomic DNA from trace amounts of starting material.

The GenomePlex® WGA kits utilize a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex® Library molecules flanked by universal priming sites. The OmniPlex® library is then amplified using universal oligonucleotide primers and a limited number of cycles. The GenomePlex® WGA kits require nanogram quantities of genomic DNA with yields of 5-10 µg following PCR. This system is suitable with a variety of purified DNA samples including whole blood, blood cards, buccal swabs, plant sources, and formalin-fixed, paraffin-embedded tissue samples.

Single Cell Whole Genome Amplification Strategies

The GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) is designed to amplify the genome of a single cell resulting in a million-fold amplification yielding microgram quantities of genomic DNA. Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. However, the GenomePlex® technology provides amplification efficiency with WGA DNA Polymerase. The Single Cell WGA kit includes all the reagents necessary for cell lysis and subsequent whole genome amplification.

The Single Cell WGA kit has been validated with numerous downstream applications and is suitable for amplification from a variety of sources including plant, polycarbonate fixed cells, lymphocytes, renal cancer cells, epithelial cells, leukemia cells, and fibroblast amniotic cells. The amplified genome is compatible with many downstream applications including comparative genomic hybridization (CGH), microsatellite/STR analysis, SNP analysis, QPCR, and other genotyping analysis.

Whole Transcriptome Amplification

Whole transcriptome amplification (WTA) kits have been developed to generate sufficient transcript targets from minute amounts of RNA. Successful WTA requires accurate replication of representative transcripts present in a sample – without dropout or bias of specific mRNAs.  Our WTA kits provide an accurate, fast and simple method of amplifying total RNA from a variety of sources including blood, fixed and frozen tissue, cell culture, FACS sorted cells, plants and microorganisms. Additionally, the WTA (WTA1WTA2SeqR) kits provide rapid amplification of total RNA in less than 4 hours without 3’-bias. Amplified RNA (cDNA) is suitable for qPCR, micro array analysis, and traditional cloning. For additional information, please visit our WTA1WTA2, or SeqR product pages.

SeqPlex-I DNA and RNA Amplification Kits for Illumina® Next Generation Sequencing

The SeqPlex-I DNA and RNA Amplification Kits for whole genome amplification (WGA) and whole transcriptome amplification (WTA) are designed to facilitate Illumina® next-generation sequencing (NGS) from extremely small quantities or degraded/highly fragmented DNA and RNA. Both the WGA and WTA kits utilize a proprietary technology based on random priming of genomic DNA or RNA and conversion of the resulting small fragments to PCR amplifiable library molecules flanked by universal priming sites. The SeqPlex-I WGA and WTA kits allow easy amplification of limited starting samples for convenient direct input onto Illumina® NGS flow cells, enabling you to accelerate your next big discovery.



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