Our pharma microbiology testing device validation protocols provide correct documentation for audits and with regulatory authorities. When we are on-site, our validation engineers ask the right questions, understand your application, and the validation requirements of your equipment.
Whole-genome TRC shRNA libraries, gene family sets or individual RNAi clones/vectors are available as glycerol stocks. Sigma-Aldrich® answers your frequently asked questions about using shRNA in this format
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Efficient qPCR relies on good assay design. Since the invention of PCR, many parameters have been identified as important for assay quality, such as estimates of oligo temperature characteristics, GC content and folding properties.
Graphene is a one-atomic-layer thick two-dimensional material made of carbon atoms arranged in a honeycomb structure. Its fascinating electrical, optical, and mechanical properties ignited enormous interdisciplinary interest from the physics, chemistry, and materials science fields.
Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.
RAFT (Reversible Addition Fragmentation chain Transfer) polymerization is a reversible deactivation radical polymerization (RDRP) and one of the more versatile methods for providing living characteristics to radical polymerization.
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or
The CRISPR/Cas9 system has recently emerged as a highly specific, efficient, and versatile gene editing technology that can be utilized to build disease models and correct diseased genes. Safe and effective delivery vectors for the CRISPR/Cas9 system are in critical
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