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SMCxPRO™ Immunoassay Instrument and SMC™ Kits
High Sensitivity Protein Measurement at the fg/mL level Synonym: SMC, Singulex, single molecule counting, xPRO
Processing Small Tissue or Cell Samples using Sample Grinding Kit
Sample Grinding Kit from GE Healthcare is designed to disrupt cell or tissue samples for protein extraction.
HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
Pharma Microbiology Testing Device Validation
Our pharma microbiology testing device validation protocols provide correct documentation for audits and with regulatory authorities. When we are on-site, our validation engineers ask the right questions, understand your application, and the validation requirements of your equipment.
Asia Pacific
Asia Pacific Region
Americas
Americas region
Mass Spectrometric Approaches to Lipidomic Studies
Dr. Robert Murphy, professor at University of Colorado - Denver, discusses Mass Spectrometric Approaches to Lipidomic Studies.
Organic Bioelectronic Materials and Devices for Bridging Biology and Traditional Electronics
Professor Rivnay (Northwestern University, USA) discusses using organic mixed conductors as an alternative to efficiently bridge the ionic world of biology with contemporary microelectronics.
Cloning Genes-of-Interest into a Plasmid Vector
This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.
RNAi Glossary
A glossary of common RNAi terms including, antisense molecules, functional genomics, gene silencing, knock-down, pseudotype, shRNA and more.
Formats — Glycerol FAQs
Whole-genome TRC shRNA libraries, gene family sets or individual RNAi clones/vectors are available as glycerol stocks. Sigma-Aldrich® answers your frequently asked questions about using shRNA in this format
Polydiacetylene Nanotubes (PDNTs): Nanotubes Based On Lipid Diacetylene Materials
Polydiacetylene nanotubes (PDNTs) are self-assembled diacetylene nanotubes comprised of cross-linked, conjugated double and triple bonds.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Quantitative PCR Basics
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
OligoArchitect™ Assay Design
Efficient qPCR relies on good assay design. Since the invention of PCR, many parameters have been identified as important for assay quality, such as estimates of oligo temperature characteristics, GC content and folding properties.
Graphene Nanoribbons: Production and Applications
Graphene is a one-atomic-layer thick two-dimensional material made of carbon atoms arranged in a honeycomb structure. Its fascinating electrical, optical, and mechanical properties ignited enormous interdisciplinary interest from the physics, chemistry, and materials science fields.
Manufacturing, Characterization and Use of Single Walled Carbon Nanotubes
Manufacturing, Characterization and Use of Single Walled Carbon Nanotubes
Whole Genome Amplification for Single Cell Biology
The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level.
Nanofluids for Biomedical Applications
Currently, magnetic nanoparticles (MNPs) are attracting a lot of attention because of the possibility of many novel applications, especially in biomedical research.
Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR
Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.
Kinetic Energy Harvesting by Magnetostrictive Materials
A significant limiting factor for wearable electronics and wireless sensors is the finite amount of energy that can be stored in on-board batteries.
Synthesis, Properties, and Applications of Perovskite-Phase Metal Oxide Nanostructures
Synthesis, Properties, and Applications of Perovskite-Phase Metal Oxide Nanostructures
Reversible Addition Fragmentation Chain Transfer (RAFT) Polymerization
RAFT (Reversible Addition Fragmentation chain Transfer) polymerization is a reversible deactivation radical polymerization (RDRP) and one of the more versatile methods for providing living characteristics to radical polymerization.
Array CGH Analysis of Challenging Samples
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or
Recent Progress on Nonviral Delivery Carriers for CRISPR/Cas9 Systems
The CRISPR/Cas9 system has recently emerged as a highly specific, efficient, and versatile gene editing technology that can be utilized to build disease models and correct diseased genes. Safe and effective delivery vectors for the CRISPR/Cas9 system are in critical
ThermaStop™ PCR Additive
PCR additive added to master mix that stops nonspecific bands after cool down.
Blue-White Screening & Protocols for Colony Selection
Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.
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