Optimizing methodologies for PCR-based DNA methylation analysis.

BioTechniques (2013-10-11)
Hernán G Hernández, M Yat Tse, Stephen C Pang, Humberto Arboleda, Diego A Forero
ABSTRACT

Comprehensive analysis of DNA methylation patterns is critical for understanding the molecular basis of many human diseases. While hundreds of PCR-based DNA methylation studies are published every year, the selection and implementation of appropriate methods for these studies can be challenging for molecular genetics researchers not yet familiar with methylation analysis. Here we review the most commonly used PCR-based DNA methylation analysis techniques: bisulfite sequencing PCR (BSP), methylation specific PCR (MSP), MethyLight, and methylation-sensitive high resolution melting (MS-HRM). We provide critical analysis of the strengths and weaknesses of each approach as well as a series of guidelines to assist in selecting and implementing an appropriate method.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Imprint® DNA Modification Kit, For bisulfite DNA conversion & purification
Sigma-Aldrich
CpGenome Turbo Bisulfite Modification Kit, The CpGenome Turbo Bisulfite Modification Kit is designed to simplify & streamline the bisulfite modification process. In just 90 minutes go from DNA sample to bisulfite converted DNA ready for analysis.