Merck

Effects and Mechanism of Chlorogenic Acid on Weight Loss.

Current pharmaceutical biotechnology (2020-03-20)
Yanchun Zhong, Yueling Ding, Laiqing Li, Meina Ge, Guangguo Ban, Hongxia Yang, Jun Dai, Licheng Zhang
ABSTRACT

Chlorogenic Acid (CA) has diverse, recognized health effects. This study aimed to explore the effects of CA on fat reduction and the underlying mechanism of these effects. First, we established a Monosodium Glutamate (MSG)-induced obesity mouse model and subjected the mice to 4 weeks of CA gavage. Then, we established an oleic acidinduced model of human fatty liver in HepG2 cells, and administered a CA intervention to the cells for 48 h. Finally, we used Oil red O staining, biochemical detection kits, RT-PCR and Western blot analysis to evaluate the effects of CA on fat reduction and on related pathways. The CA treatment could reduce fat accumulation in the liver and reduce blood lipid levels. In addition, CA decreased the mRNA and protein levels of peroxisome proliferator-activated receptor gamma, coactivator 1 α (PGC-1α) and Uncoupling Protein 1 (UCP1) in the MSG-induced obesity mouse model and the oleic acid-induced HepG2 cells. Based on the above results, we deduced that CA could reduce body weight and fat deposition in vitro and in vivo and that the mechanism may be related to the PGC-1α/UCP-1 pathway. CA can be developed as a drug to lower blood lipids and to treat obesity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)