Merck
  • Home
  • Search Results
  • Glycogen and Extracellular Glucose Estimation from Cyanobacteria Synechocystis sp. PCC 6803.

Glycogen and Extracellular Glucose Estimation from Cyanobacteria Synechocystis sp. PCC 6803.

Bio-protocol (2018-05-05)
Md Rezaul Islam Khan, Yushu Wang, Shajia Afrin, Lin He, Gang Ma
ABSTRACT

Cyanobacteria, which have the extraordinary ability to grow using sunlight and carbon dioxide, are emerging as a green host to produce value-added products. Exploitation of this highly promising host to make products may depend on the ability to modulate the glucose metabolic pathway; it is the key metabolic pathway that generates intermediates that feed many industrially important pathways. Thus, before cyanobacteria can be considered as a leading source to produce value-added products, we must understand the interaction between glucose metabolism and other important cellular activities such as photosynthesis and chlorophyll metabolism. Here we describe reproducible and reliable methods for measuring extracellular glucose and glycogen levels from cyanobacteria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium acetate, anhydrous, for molecular biology, ≥99%
Supelco
D-(+)-Glucose solution, 1 mg/mL in 0.1% benzoic acid, standard for enzymatic assay kits GAGO20, GAHK20, STA20, analytical standard