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Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin.

Journal of cellular physiology (1989-08-01)
T Kitamura, T Tange, T Terasawa, S Chiba, T Kuwaki, K Miyagawa, Y F Piao, K Miyazono, A Urabe, F Takaku

We have established a novel cell line, designated as TF-1, from a patient with erythroleukemia, which showed complete growth dependency on granulocyte-macrophage colony-stimulating factor (GM-CSF) or on interleukin-3 (IL-3) and carried a homogeneous chromosomal abnormality (54X). Erythropoietin (EPO) also sustained the short-term growth of TF-1, but did not induce erythroid differentiation. These three hematopoietic growth factors acted on TF-1 synergistically. Transforming growth factor-beta and interferons inhibited the factor-dependent growth of TF-1 cells in a dose-dependent fashion, and monocyte-colony stimulating factor and interkeukin-1 enhanced the GM-CSF-dependent growth of TF-1. Ultrastructural studies revealed some very immature features in this cell line. Although TF-1 cells do not express glycophorin A or carbonyl anhydrase I, the morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of TF-1 to erythroid lineage. When induced to differentiate, TF-1 entered two different pathways. Specifically, hemin and delta-aminolevulinic acid induced hemoglobin synthesis, whereas TPA induced dramatic differentiation of TF-1 into macrophage-like cells. In summary, TF-1 is a cell line of immature erythroid origin that requires GM-CSF, IL-3, or EPO for its growth and that has the ability to undergo differentiation into either more mature erythroid cells or into macrophage-like cells. TF-1 is a useful tool for analyzing the human receptors for IL-3, GM-CSF, and EPO or the signal transduction of these hemopoietic growth factors.

Product Number
Product Description

Oncostatin M human, BioReagent, ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Erythropoietin Soluble Receptor human, >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
Erythropoietin from rat, recombinant, expressed in insect cells, suitable for cell culture
TF1 Cell Line human, Erythroleukemic cell line from blood., 93022307
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture
Monoclonal Anti-Oncostatin M antibody produced in mouse, clone 17001, purified immunoglobulin, lyophilized powder