Merck
  • Home
  • Search Results
  • Isolation and characterization of novel sulfate-reducing bacterium capable of anaerobic degradation of p-xylene.

Isolation and characterization of novel sulfate-reducing bacterium capable of anaerobic degradation of p-xylene.

Microbes and environments (2012-03-27)
Yuriko Higashioka, Hisaya Kojima, Manabu Fukui
ABSTRACT

A novel strain of p-xylene-degrading sulfate reducer was isolated in pure culture. Strain PP31 was obtained from a p-xylene-degrading enrichment culture established from polluted marine sediment. Analyses of the 16S rRNA gene and two functional genes involved in sulfate respiration and anaerobic degradation of aromatic compounds revealed that the isolate was closely related to members of the genus Desulfosarcina. Strain PP31 was capable of growing on p-xylene under sulfate-reducing conditions, and the ratio of generated sulfide and consumed p-xylene suggested complete oxidation by the novel isolate. The strain could not grow on benzene, toluene, ethylbenzene, m-xylene o-xylene, or n-hexane as an electron donor. Strain PP31 is the first isolated bacterium that degrades p-xylene anaerobically, and will be useful to understanding the mechanism of anaerobic degradation of p-xylene.

MATERIALS
Product Number
Brand
Product Description

Supelco
p-Xylene, analytical standard
Sigma-Aldrich
p-Xylene, anhydrous, ≥99%
Supelco
p-Xylene solution, certified reference material, 5000 μg/mL in methanol
Supelco
p-Xylene, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
p-Xylene, suitable for HPLC, ≥99%
Sigma-Aldrich
p-Xylene, puriss. p.a., ≥99.0% (GC)
Sigma-Aldrich
p-Xylene, ReagentPlus®, 99%