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Screening and analyzing the potential bioactive components from reduning injection, using macrophage cell extraction and ultra-high performance liquid chromatography coupled with mass spectrometry.

The American journal of Chinese medicine (2013-01-23)
Yonghui Li, Peixiang Wang, Wei Xiao, Li Zhao, Zhenzhong Wang, Li Yu
ABSTRACT

In this paper, a useful method for screening and analyzing the potential bioactive components from Reduning Injection was developed using macrophage cell extraction and ultra-high performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on macrophage cell damage induced by LPS in vitro were also investigated. The results showed that chlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid significantly inhibited the prostaglandin E(2) (PGE(2)) release and reversed the interleukin-6 (IL-6) secretion of macrophages (p < 0.05). These data indicated that the method of macrophage cell extraction coupled with UPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCM injection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Chlorogenic acid, primary reference standard