Merck

Action of chlorogenic acid on the complement system.

Anais da Academia Brasileira de Ciencias (1999-07-21)
R Ejzemberg, M H Da Silva, L Pinto, W B Mors
ABSTRACT

Previous research on plants used in folk medicine as antidotes against snake-bite revealed some constituents responsible for such protection. Chlorogenic acid (3-0-caffeoyl quinic acid) was one of these substances, studied with more attention. It has been shown that this substance binds to proteins through hydrophobic interactions and hydrogen bonds. This paper shows the preliminary results about the anti-complementary action of chlorogenic acid. Human and guinea pig sera, treated with chlorogenic acid, were added to the hemolytic system (sheep erythrocyte sensitized with hemolysin) to study its effect on the activation of the classical complement pathway. The action on the alternative pathway was studied with human serum treated with chlorogenic acid and zymosan. Our results show that chlorogenic acid presents anti-complementary action at the classical pathway, since the sera are not able to lysis the indicator system. The presence of C3b fragments on the surface of the yeast cells demonstrates that the alternative pathway was not affected.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Chlorogenic acid, primary reference standard