Merck
  • Home
  • Search Results
  • Concentrations of riboflavin and related organic acids in children with protein-energy malnutrition.

Concentrations of riboflavin and related organic acids in children with protein-energy malnutrition.

The American journal of clinical nutrition (2000-03-25)
C D Capo-Chichi, F Feillet, J L Guéant, K Amouzou, N Zonon, A Sanni, E Lefebvre, K Assimadi, M Vidailhet
ABSTRACT

Riboflavin, flavin mononucleotide (FMN), and flavin adenine dinucleotide (FAD) concentrations have been little studied in cases of malnutrition. Our objective was to investigate the effects of malnutrition on riboflavin status and riboflavin's relation with thyroid hormones and concentrations of urinary organic acids. Malnourished children from the savannah in Benin (group S, n = 30) and the coast in Togo (group C, n = 30), as well as 24 control subjects from both regions, were studied. Blood riboflavin, FMN, and FAD were analyzed by HPLC; urinary organic acids were analyzed by gas chromatography-mass spectrometry. Children in group S were more severely malnourished than children in group C. Triiodothyronine concentrations were lower in group S than in group C or the control group (1.12 +/- 0.24 compared with 1.74 +/- 0.18 and 2.92 +/- 0.19 nmol/L, respectively; P < 0.0001). Plasma riboflavin concentrations in group S were higher than those in group C or the control group (66.90 +/- 12.75 compared with 28.09 +/- 9.12 and 20.08 +/- 3.03 nmol/L, respectively; P < 0.001). Plasma FAD concentrations in group S were lower than those in group C or the control group (31.57 +/- 10.19 compared with 59.02 +/- 5.60 and 65.35 +/- 5.23 nmol/L, respectively; P < 0.0001). Dicarboxylic aciduria was higher in group C than in group S or the control subjects. Children in group S had low triiodothyronine concentrations and low conversion of plasma riboflavin into its cofactors, leading to a plasma FAD deficiency. Plasma FAD was not correlated with urinary dicarboxylic acid concentrations.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Thyroxine, ≥98% (HPLC)
Sigma-Aldrich
3,3′,5-Triiodo-L-thyronine, ≥95% (HPLC), powder
Sigma-Aldrich
L-Thyroxine, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Methylsuccinic acid, 99%
Sigma-Aldrich
Glutaric acid, 99%
Sigma-Aldrich
Pimelic acid, 98%
Supelco
Glutaric acid, certified reference material, TraceCERT®
Sigma-Aldrich
Succinic acid, ReagentPlus®, BioRenewable, ≥99.0%
Sigma-Aldrich
Succinic acid, BioXtra, BioRenewable, ≥99.0%
Sigma-Aldrich
Succinic acid, BioReagent, BioRenewable, suitable for cell culture, suitable for insect cell culture
Supelco
L-Thyroxine (T4) solution, 100 μg/mL in methanol with 0.1N NH3, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
3,3′,5-Triiodo-L-thyronine (T3) solution, 100 μg/mL in methanol with 0.1N NH3, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
Succinic acid, certified reference material, TraceCERT®
Supelco
Adipic acid, certified reference material, TraceCERT®
Sigma-Aldrich
Succinic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
Adipic acid, ≥99.5%
Sigma-Aldrich
Suberic acid, 98%
Sigma-Aldrich
Ethylmalonic acid, 97%
Sigma-Aldrich
Succinic acid, puriss. p.a., ACS reagent, ≥99.5% (T)
Sigma-Aldrich
Succinic acid, purum p.a., ≥99.0% (T)
Sigma-Aldrich
Adipic acid, 99%
Sigma-Aldrich
Adipic acid, BioXtra, ≥99.5% (HPLC)
Sigma-Aldrich
Suberic acid, purum, ≥98.0% (T)