Availability of certain monosaccharides in the composition of glycocalyx of lactic acid bacteria (Lactobacillus plantarum--strains 337D and 11/16; Streptococcus thermophilus--strains S1 (nonmucous race) and S5 (mucous race), Enterococcus faecium (K-50) has been investigated with the help of plant lectins with certain carbohydrate specificity labelled by colloid gold. All the microorganisms under investigation were characterized by the presence of N-acetyl-D-galactosamine and N-acetyl-D-glucosamine in rather insignificant amounts. Glycocalyx of lactic acid bacteria was also characterized by availability of essential amount of L-fructose and low amount of sialic acid (except for S. thermophilus S5 (mucous race). Presence of alpha-N-acetyl-D-galactosamine, alpha-D, beta-D-galactose, alpha-D-glucose, alpha-D-mannose in the composition of the lactic acid bacteria glycocalyx composition evidences for the additional role of these monosaccharides in the process of the microorganism adhesion on the human and animal intestine mucosa. It has been confirmed that availability of certain monosaccharides in the composition of surface glycopolymers of lactic acid bacteria was connected with adhesive properties of cells and their existence conditions.