Merck
  • Home
  • Search Results
  • Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Clinical chemistry (1989-04-01)
G Hoffmann, S Aramaki, E Blum-Hoffmann, W L Nyhan, L Sweetman
ABSTRACT

This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Citric acid, 99%
Sigma-Aldrich
L-(+)-Lactic acid, BioXtra, ≥98% (titration)
Sigma-Aldrich
Citric acid, anhydrous, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
Oleic acid, suitable for cell culture, BioReagent
Sigma-Aldrich
L-(−)-Malic acid, suitable for cell culture, BioReagent, suitable for insect cell culture
Sigma-Aldrich
L-(−)-Malic acid, ≥95% (titration)
Sigma-Aldrich
L-(−)-Malic acid, BioXtra, ≥95%
Sigma-Aldrich
Oleic acid, ≥99% (GC)
Sigma-Aldrich
Azelaic acid, 98%
Sigma-Aldrich
Fumaric acid, FCC, FG
Sigma-Aldrich
4-Hydroxybenzoic acid, ReagentPlus®, ≥99%
Sigma-Aldrich
Benzoic acid, ACS reagent, ≥99.5%
Sigma-Aldrich
4-Hydroxybenzoic acid, ReagentPlus®, 99%
Sigma-Aldrich
Pyruvic acid, ≥97%, FG
Sigma-Aldrich
Stearic acid, reagent grade, 95%
Sigma-Aldrich
Pyruvic acid, natural, ≥95%, FG
Sigma-Aldrich
L-(−)-Malic acid, 97%, optical purity ee: 99% (GLC)
Sigma-Aldrich
Oleic acid, natural, FCC
Sigma-Aldrich
Stearic acid, Grade I, ≥98.5% (capillary GC)
Sigma-Aldrich
L-(+)-Lactic acid, ≥98%
Sigma-Aldrich
Pyruvic acid, 98%
Sigma-Aldrich
Citric acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Azelaic acid, technical grade, 80%
Sigma-Aldrich
Pyruvic acid, 95%
Sigma-Aldrich
Succinic acid, puriss. p.a., ACS reagent, ≥99.5% (T)
Sigma-Aldrich
Azelaic acid, technical, ~85% (GC)
Sigma-Aldrich
Succinic acid, purum p.a., ≥99.0% (T)
Sigma-Aldrich
L-(+)-Tartaric acid, ACS reagent, ≥99.5%
Sigma-Aldrich
Benzoic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Succinic acid, ACS reagent, ≥99.0%