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  • Grid-layout and theta-modulation of layer 2 pyramidal neurons in medial entorhinal cortex.

Grid-layout and theta-modulation of layer 2 pyramidal neurons in medial entorhinal cortex.

Science (New York, N.Y.) (2014-01-25)
Saikat Ray, Robert Naumann, Andrea Burgalossi, Qiusong Tang, Helene Schmidt, Michael Brecht
ABSTRACT

Little is known about how microcircuits are organized in layer 2 of the medial entorhinal cortex. We visualized principal cell microcircuits and determined cellular theta-rhythmicity in freely moving rats. Non-dentate-projecting, calbindin-positive pyramidal cells bundled dendrites together and formed patches arranged in a hexagonal grid aligned to layer 1 axons, parasubiculum, and cholinergic inputs. Calbindin-negative, dentate-gyrus-projecting stellate cells were distributed across layer 2 but avoided centers of calbindin-positive patches. Cholinergic drive sustained theta-rhythmicity, which was twofold stronger in pyramidal than in stellate neurons. Theta-rhythmicity was cell-type-specific but not distributed as expected from cell-intrinsic properties. Layer 2 divides into a weakly theta-locked stellate cell lattice and spatiotemporally highly organized pyramidal grid. It needs to be assessed how these two distinct principal cell networks contribute to grid cell activity.

MATERIALS
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