• Home
  • Search Results
  • Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation.

Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation.

Methods in molecular biology (Clifton, N.J.) (2006-05-25)
David J Waxman, Thomas K H Chang
ABSTRACT

A spectrofluorometric method is described for the determination of CYP2A6-catalyzed coumarin 7-hydroxylation. Following acidification of the reaction mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hydroxylation activity in microsomes prepared from liver and other tissues and in isolated hepatocytes and cultured cells that express this cytochrome P450 activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
β-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate