Merck
  • Home
  • Search Results
  • Fructose- and glucose-conditioned preferences in FVB mice: strain differences in post-oral sugar appetition.

Fructose- and glucose-conditioned preferences in FVB mice: strain differences in post-oral sugar appetition.

American journal of physiology. Regulatory, integrative and comparative physiology (2014-10-17)
Anthony Sclafani, Steven Zukerman, Karen Ackroff
ABSTRACT

Recent studies indicate that, unlike glucose, fructose has little or no post-oral preference conditioning actions in C57BL/6J (B6) mice. The present study determined whether this is also the case for FVB mice, which overconsume fructose relative to B6 mice. In experiment 1, FVB mice strongly preferred a noncaloric 0.1% sucralose + 0.1% saccharin (S+S) solution to 8% fructose in a 2-day choice test but switched their preference to fructose after separate experience with the two sweeteners. Other FVB mice displayed a stronger preference for 8% glucose over S+S. In a second experiment, ad libitum-fed FVB mice trained 24 h/day acquired a significant preference for a flavor (CS+) paired with intragastric (IG) self-infusions of 16% fructose over a different flavor (CS-) paired with IG water infusions. IG fructose infusions also conditioned flavor preferences in food-restricted FVB mice trained 1 h/day. IG infusions of 16% glucose conditioned stronger preferences in FVB mice trained 24- or 1 h/day. Thus, fructose has post-oral flavor conditioning effects in FVB mice, but these effects are less pronounced than those produced by glucose. Further studies of the differential post-oral conditioning effects of fructose and glucose in B6 and FVB mice should enhance our understanding of the physiological processes involved in sugar reward.

MATERIALS
Product Number
Brand
Product Description

Sucralose, European Pharmacopoeia (EP) Reference Standard
Supelco
Saccharin, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Sucralose, United States Pharmacopeia (USP) Reference Standard
Supelco
Sucralose, Pharmaceutical Secondary Standard; Certified Reference Material
Saccharin, European Pharmacopoeia (EP) Reference Standard
USP
Saccharin, United States Pharmacopeia (USP) Reference Standard
Supelco
Sucralose, analytical standard
Sigma-Aldrich
Sucralose, ≥98.0% (HPLC)
Sigma-Aldrich
Saccharin, ≥98%
Sigma-Aldrich
Saccharin, ≥99%
Supelco
Ethyl acetate, analytical standard
Millipore
Sucrose, ACS reagent, suitable for microbiology, ≥99.0%
Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
Supelco
Propyl acetate, analytical standard
Supelco
Sucrose, analytical standard, for enzymatic assay kit SCA20
Sigma-Aldrich
Sucrose, meets USP testing specifications
Sigma-Aldrich
Sucrose, ≥99.5%
Sigma-Aldrich
Sucrose, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, Grade I, suitable for plant cell culture
Sigma-Aldrich
Sucrose, ACS reagent
Sigma-Aldrich
Sucrose, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, Grade II, suitable for plant cell culture
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sigma-Aldrich
Propyl acetate, ≥98%, FG
Sigma-Aldrich
Ethyl acetate, natural, ≥99%, FCC, FG
Sigma-Aldrich
Ethyl acetate, ≥99%, FCC, FG
Sigma-Aldrich
Propyl acetate, 99%
Sigma-Aldrich
Ethyl acetate, anhydrous, 99.8%
Supelco
Sodium Saccharin, analytical standard