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Whole-exome sequencing identifies somatic ATRX mutations in pheochromocytomas and paragangliomas.

Nature communications (2015-01-22)
Lauren Fishbein, Sanika Khare, Bradley Wubbenhorst, Daniel DeSloover, Kurt D'Andrea, Shana Merrill, Nam Woo Cho, Roger A Greenberg, Tobias Else, Kathleen Montone, Virginia LiVolsi, Douglas Fraker, Robert Daber, Debbie L Cohen, Katherine L Nathanson
ABSTRACT

Pheochromocytomas and paragangliomas (PCC/PGL) are the solid tumour type most commonly associated with an inherited susceptibility syndrome. However, very little is known about the somatic genetic changes leading to tumorigenesis or malignant transformation. Here we perform whole-exome sequencing on a discovery set of 21 PCC/PGL and identify somatic ATRX mutations in two SDHB-associated tumours. Targeted sequencing of a separate validation set of 103 PCC/PGL identifies somatic ATRX mutations in 12.6% of PCC/PGL. PCC/PGL with somatic ATRX mutations are associated with alternative lengthening of telomeres and clinically aggressive behaviour. This finding suggests that loss of ATRX, an SWI/SNF chromatin remodelling protein, is important in the development of clinically aggressive PCC/PGL.

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