• Home
  • Search Results
  • Trypanocidal activity of free and nanoencapsulated curcumin on Trypanosoma evansi.

Trypanocidal activity of free and nanoencapsulated curcumin on Trypanosoma evansi.

Parasitology (2014-08-20)
L T Gressler, C B Oliveira, K Coradini, L Dalla Rosa, T H Grando, M D Baldissera, C E Zimmermann, A S Da Silva, T C Almeida, C L Hermes, P Wolkmer, C B Silva, K L S Moreira, R C R Beck, R N Moresco, M L Da Veiga, L M Stefani, S G Monteiro
ABSTRACT

This study aimed to evaluate in vitro and in vivo trypanocidal activity of free and nanoencapsulated curcumin against Trypanosoma evansi. In vitro efficacy of free curcumin (CURC) and curcumin-loaded in lipid-core nanocapsules (C-LNCs) was evaluated to verify their lethal effect on T. evansi. To perform the in vivo tests, T. evansi-infected animals were treated with CURC (10 and 100 mg kg(-1), intraperitoneally [i.p.]) and C-LNCs (10 mg kg(-1), i.p.) during 6 days, with the results showing that these treatments significantly attenuated the parasitaemia. Infected untreated rats showed protein peroxidation and an increase of nitrites/nitrates, whereas animals treated with curcumin showed a reduction on these variables. As a result, the activity of antioxidant enzymes (superoxide dismutase and catalase) differs between groups (P<0.05). Infected animals and treated with CURC exhibited a reduction in the levels of alanine aminotransferase and creatinine, when compared with the positive control group. The use of curcumin in vitro resulted in a better parasitaemia control, an antioxidant activity and a protective effect on liver and kidney functions of T. evansi-infected adult male Wistar rats.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Curcumin, from Curcuma longa (Turmeric), powder
Sigma-Aldrich
Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Curcumin, ≥94% (curcuminoid content), ≥80% (Curcumin)
Sigma-Aldrich
Acetone, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.8%
Supelco
Acetone, analytical standard
Supelco
Curcumin, analytical standard
Supelco
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, histological grade, ≥99.5%
Sigma-Aldrich
Acetone, natural, ≥97%
Supelco
Curcumin, matrix substance for MALDI-MS, ≥99.5% (HPLC)
Supelco
Acetone solution, certified reference material, 2000 μg/mL in methanol: water (9:1)
Sigma-Aldrich
Sorbitan monostearate, meets FCC analytical specifications
USP
Acetone, United States Pharmacopeia (USP) Reference Standard
USP
Curcumin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Acetone, ≥99%, meets FCC analytical specifications
Curcumin, primary reference standard
Sigma-Aldrich
Acetone, purum, ≥99.0% (GC)