Merck

Analysis of Phenolic Compounds in Some Medicinal Herbs by LC-MS.

Journal of chromatographic science (2015-01-15)
Alina O Matei, Florentina Gatea, Gabriel L Radu
ABSTRACT

In this paper, a liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry in negative mode method was developed for the identification and quantitative determination of 13 individual phenolics (chlorogenic acid, caffeic acid, coumaric acid, ferulic acid, (+)-catechin, (-)-epicatechin, rutin, quercitrin, isoquercitrin, fisetin, isorhamnetin, hesperidin and chrysin) from ethanolic extracts [30, 50 and 70% (w/v)] of Calendula officinalis, Hypericum perforatum, Galium verum and Origanum vulgare and some commercial extracts of these medicinal herbs. Correlation coefficients (r(2)) from calibration curves for all the compounds were between 0.9971 and 0.9996. Limit of detection was in the range of 0.070-0.280 µg/mL and limit of quantification was from 0.233 to 0.932 µg/mL. The method was partially validated and the results obtained are: the intra- and interday relative standard deviation values were within 0.086 and 2.821% and recovery values vary from 95.84% (coumaric acid) to 103.20% (rutin).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Sigma-Aldrich
(−)-Epicatechin, ≥98% (HPLC), from green tea
Sigma-Aldrich
(−)-Epicatechin, ≥90% (HPLC)
Sigma-Aldrich
Quercetin 3-β-D-glucoside, ≥90% (HPLC)
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Sigma-Aldrich
Caffeic acid, ≥98.0% (HPLC)
Sigma-Aldrich
Hesperidin, ≥80%
Sigma-Aldrich
Acetonitrile, for DNA synthesis
Sigma-Aldrich
Ultrapure Acetonitrile, for DNA synthesis
Sigma-Aldrich
Acetonitrile, configured for PerkinElmer 8900, configured for Polygen, for DNA synthesis
Sigma-Aldrich
Formic acid, ≥95%, FCC, FG
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
Formic acid, ACS reagent, ≥96%
Sigma-Aldrich
Acetonitrile
Sigma-Aldrich
Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)
Sigma-Aldrich
Acetonitrile, biotech. grade, ≥99.93%
Sigma-Aldrich
Formic acid, ACS reagent, ≥88%
Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetonitrile, ≥99.5% (GC)
Sigma-Aldrich
Acetonitrile, for preparative HPLC, ≥99.8% (GC)
Sigma-Aldrich
Formic acid, reagent grade, ≥95%
Sigma-Aldrich
Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
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Acetonitrile, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5% (GC)
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Formic acid, puriss., meets analytical specifications of DAC, FCC, 98.0-100%
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Ultrapure Acetonitrile
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Acetonitrile, anhydrous, 99.8%
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Acetonitrile, for DNA synthesis
Sigma-Aldrich
trans-Ferulic acid, 99%
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trans-Ferulic acid, ≥99%