Merck
  • Home
  • Search Results
  • Ascorbic acid in lymphocytes: cell preparation and liquid-chromatographic assay.

Ascorbic acid in lymphocytes: cell preparation and liquid-chromatographic assay.

Clinical chemistry (1982-10-01)
W Lee, P Hamernyik, M Hutchinson, V A Raisys, R F Labbé
ABSTRACT

Measurements of ascorbic acid concentration in leukocytes by "high-performance" liquid chromatography (HPLC) provides better nutritional assessment, leading to better management, particularly of presymptomatic and critically ill patients. This procedure includes a simple, reproducible cell-separation technique that requires no more than 2 mL of whole blood. Cell populations are separable with greater than 95% purity and greater than 99% viability. Ascorbic acid is assayed by HPLC. The vitamin can be reproducibly quantified in concentrations as low as 0.1 microgram/mL of cell extract. The chromatographic procedure is very rapid, analysis being completed within 15 min after specimen preparation. The assay is suitable also for urine and protein-free filtrates of plasma and of other biological materials. Reference intervals for plasma, mononuclear leukocytes, and polymorphonuclear leukocytes were established. A preliminary clinical evaluation revealed that hospital patients were at a greater risk of ascorbic acid deficiency than expected.

MATERIALS
Product Number
Brand
Product Description

USP
Ascorbic acid, United States Pharmacopeia (USP) Reference Standard