Amersham ECL, Amersham ECL Prime, and Amersham ECL Select detection systems require very little antibody to achieve a sufficient sensitivity; therefore, the amount of antibody (primary and secondary) used in the protocols can be minimized. Smaller quantities of antibody-buffer mixtures can be used by scaling down the protocol and performing the incubations in sealable plastic bags.
Figure 4.4 shows typical Western blot results using Anti-GST Antibody.
Figure 4.4.Western blot of E. coli lysates containing GST-tagged proteins. For detection, Anti-GST Antibody, anti-goat IgG alkaline phosphatase conjugate, and DNB/nitro-blue tetrazolium chloride (NBT) enzyme substrate were used.
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