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Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Toolkit für erneuerbare Elektrizität

Das Toolkit für Anbieter von erneuerbarem Strom hilft Anbietern, die Grundlagen der erneuerbaren Energien zu erlernen und Projekte in die Tat umzusetzen.

Toolkit per l'elettricità rinnovabile

Il Toolkit per i fornitori di energia elettrica rinnovabile aiuta i fornitori a conoscere le basi dell'energia elettrica rinnovabile e a realizzare i progetti.

Renewable Electricity Toolkit

The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

PCR/qPCR/dPCR Assay Design

PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Dendritic Polyester Scaffolds for Drug Delivery

Dendritic molecules' biomedical applications, particularly bis-MPA dendritic scaffolds, hold promise for drug delivery.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well ﬁlter Plates

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well ﬁlter plates from Cytiva.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Robust, load independent viral clearance in monoclonal antibody purification with Natrix® Q chromatography membrane

Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing

Manual and Automated Puriﬁcation

This page describes manual and automated puriﬁcation of histidine-tagged recombinant proteins using Cytiva products

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Optimizing Puriﬁcation of Histidine-Tagged Proteins

How to optimize puriﬁcation of histidine-tagged proteins using Cytiva products.

Peptide Impurities

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic ﬂuid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

Performing a Separation with IgG Sepharose 6 Fast Flow

Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by afﬁnity chromatography using HiTrap® Protein G HP columns from Cytiva.

Performing a Puriﬁcation and On-column Refolding of an Insoluble Histidine-tagged Protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by afﬁnity chromatography using HiTrap™ IgM Purification HP.

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