CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.


CRISPR Formats

SygRNA® Synthetic gRNA

§ Modified tracrRNA is needed when using modified crRNA.

SygRNA® synthetic CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9. When ordering sgRNA or crRNA:tracrRNA, be sure to select the species that matches your Cas9 source.

View our protocols to learn more about SygRNA® synthetic CRISPR.

Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Plasmid DNA: provided in 1xTris-EDTA

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Web ordered lentivirus clones are provided in individually labeled vials. Requests for aliquots in plates should be submitted to CRISPR@sial.com.

Sanger QuickPick™ Knockout gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time

CRISPR gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time

In Vitro Transcribed RNA (IVT): provided in Molecular Grade Water (DNase-, RNase-, Protease-free)

Cas9 Products

Click on the product number to order and see additional product details

Cas9 Proteins

Cas9 Plasmids and Lentivirus

SAM CRISPR Activator Cas9 Products

CRISPR Inhibition Cas9 Products & Controls

Vectors

Mammalian System Vectors

Lentiviral Vectors

†Web ordering is currently available in select countries. Global web ordering capabilities are in development. Please contact CRISPR@sial.com for questions or assistance placing clone orders.

Plant Vectors

‖ Web ordering is currently available in select countries.

Bacterial Editors

CRISPR Controls

Click on the product number to order and see additional product details

Volume Price Discounts

Vector Maps and Sequences

Click on the images below for a larger view.

p01 Vector

p02 Vector

p03 Vector

p04 Vector

p05 Vector

p10 Vector

p50 Vector

p51 Vector

p52 Vector

p53 Vector

p54 Vector

p55 Vector

p56 Vector

p57 Vector

p58 Vector

p59 Vector

p60 Vector

p61 Vector

p62 Vector

p63 Vector

LV01 Vector

LV02 Vector

LV03 Vector

LV04 Vector

LV05 Vector

LV06 Vector

LV07 Vector

LV17 Vector

KRAB-dCas9 Vector

Product Guarantee

We are so confident in the performance of our SygRNA® products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.

To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your SygRNA® gRNA. To receive your replacement, simply email oligotechserv@milliporesigma.com and include sample data from a representative experiment (T7E1, TIDE, or NGS)

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