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Nella SPE, uno o più analiti di un campione liquido vengono isolati mediante estrazione, partizione e/o adsorbimento su una fase stazionaria solida. * * * ## Prodotti correlati Slide 1 of 22 1 of 6 [ \ Supelco \ 575659-U \ HybridSPE®-PLus 96-Well Plate](https://www.sigmaaldrich.com/CH/it/product/supelco/575659u) Visualizzazione rapida [ \ Supelco \ 57057 \ Supelclean™ LC-Florisil® SPE Tube](https://www.sigmaaldrich.com/CH/it/product/supelco/57057) Visualizzazione rapida [ \ Supelco \ 57226 \ Supelclean™ENVI™-Chrom P SPE Tube](https://www.sigmaaldrich.com/CH/it/product/supelco/57226) Visualizzazione rapida [ \ Supelco \ 54024-U \ Supelclean™ ENVI-Carb/NH2 SPE Tube](https://www.sigmaaldrich.com/CH/it/product/supelco/54024u) Visualizzazione rapida [ \ Supelco \ 54035-U \ Supelclean™ ENVI-Carb/NH2 SPE Tube](https://www.sigmaaldrich.com/CH/it/product/supelco/54035u) Visualizzazione rapida [ \ Supelco \ 57088 \ Supelclean™ ENVI-Carb™ SPE Tube](https://www.sigmaaldrich.com/CH/it/product/supelco/57088) Visualizzazione rapida [ \ Supelco \ 57094 \ Supelclean™ ENVI-Carb™ SPE Tube](https://www.sigmaaldrich.com/CH/it/product/supelco/57094) Visualizzazione rapida [ \ Supelco \ 52738-U \ Supelclean™ PSA SPE Bulk Packing](https://www.sigmaaldrich.com/CH/it/product/supelco/52738u) Visualizzazione rapida [ \ Supelco \ 57210-U \ Supelclean™ ENVI-Carb™ SPE Bulk Packing](https://www.sigmaaldrich.com/CH/it/product/supelco/57210u) Visualizzazione rapida [ \ Supelco \ 28399-U \ Dual-Layer Carbon Reversible Tube (Micro-Column)](https://www.sigmaaldrich.com/CH/it/product/supelco/28399u) Visualizzazione rapida [ \ Supelco \ 28397-U \ Multi-Layer Silica Gel Dioxin Column](https://www.sigmaaldrich.com/CH/it/product/supelco/28397u) Visualizzazione rapida [ \ Supelco \ 57044 \ Visiprep™ SPE Vacuum Manifold](https://www.sigmaaldrich.com/CH/it/product/supelco/57044) Visualizzazione rapida [ \ Supelco \ 57265 \ Visiprep™ SPE Vacuum Manifold](https://www.sigmaaldrich.com/CH/it/product/supelco/57265) Visualizzazione rapida [ \ Supelco \ 57493-U \ Supel™ Swift HLB SPE Tubes](https://www.sigmaaldrich.com/CH/it/product/supelco/57493u) Visualizzazione rapida [ \ Supelco \ 55274-U \ Supel™Genie C8 Online SPE Starter Kit](https://www.sigmaaldrich.com/CH/it/product/supelco/55274u) Visualizzazione rapida [ \ Supelco \ 55228-U \ Supel™ QuE](https://www.sigmaaldrich.com/CH/it/product/supelco/55228u) Visualizzazione rapida [ \ Supelco \ 55227-U \ Supel™ QuE](https://www.sigmaaldrich.com/CH/it/product/supelco/55227u) Visualizzazione rapida [ \ Supelco \ 52977-U \ HybridSPE® DPX Tip](https://www.sigmaaldrich.com/CH/it/product/supelco/52977u) Visualizzazione rapida [ \ Supelco \ 575608-U \ Discovery® DSC-Si SPE 96-well Plate](https://www.sigmaaldrich.com/CH/it/product/supelco/575608u) Visualizzazione rapida [ \ Supelco \ 54341-U \ Supelclean™ EZ-POP NP](https://www.sigmaaldrich.com/CH/it/product/supelco/54341u) Visualizzazione rapida [ \ Supelco \ 55324-U \ Supel™Genie HybridSPE® Online SPE Starter Kit](https://www.sigmaaldrich.com/CH/it/product/supelco/55324u) Visualizzazione rapida [ \ Supelco \ 52974-U \ HybridSPE® DPX Tip](https://www.sigmaaldrich.com/CH/it/product/supelco/52974u) Visualizzazione rapida * * * ## Categorie in evidenza [](https://www.sigmaaldrich.com/CH/it/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [Estrazione in fase solida (SPE) e QuEChERS](https://www.sigmaaldrich.com/CH/it/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) Scopri diversi materiali per l'estrazione in fase solida (SPE) e la preparazione dei campioni QuEChERS. 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Nella SPE, uno o più analiti di un campione liquido vengono isolati mediante estrazione, partizione e/o adsorbimento su una fase stazionaria solida.   La preparazione del campione mediante SPE modifica la matrice originale di un campione in un ambiente di matrice più semplice. Ciò rende il campione più adatto alla successiva cromatografia analitica, spesso semplificando e migliorando l'analisi qualitativa e quantitativa finale. La matrice del campione più semplice riduce anche la richiesta di un sistema analitico, prolungandone potenzialmente la durata. Una procedura SPE ottimale può consentire di: - Scambiare le matrici del campione per renderle più compatibili con il metodo cromatografico di destinazione. - Concentrare gli analiti (arricchimento in tracce) per aumentare la sensibilità. - Eliminare le interferenze che causano un fondo elevato, picchi fuorvianti e/o scarsa sensibilità durante l'analisi cromatografica. - Proteggere la colonna analitica dai contaminanti. - Automatizzare il processo di estrazione. ## Come funziona la SPE? Nella SPE, la fase stazionaria (un sorbente o una resina) lega l'analita o l'impurità attraverso interazioni forti ma reversibili per estrarre in modo affidabile e rapido l'analita di interesse da un campione complesso. La SPE è selettiva e versatile, poiché sono disponibili molti sorbenti e condizioni di eluizione diversi per analiti e matrici differenti. I sorbenti SPE più comuni includono: - a base di silice - a fase inversa (C18, C8, ciano, fenile) - Fase normale (silice, diolo, NH2) - Scambio ionico (SAX, WCX, SCX) - a base di carbonio - a base di polimeri (varie composizioni, diverse funzionalità) - altri, ad esempio Florisil® (silicato di magnesio) o Allumina - Letto misto: Combinazioni di quasi tutti i precedenti in strati sequenziali ## Strategie per la SPE Nella "Bind-Elute SPE", l'analita di interesse viene catturato dal sorbente e le interferenze della matrice passano attraverso la cartuccia. In "Interference Removal SPE", le interferenze della matrice sono catturate dal sorbente e gli analiti di interesse passano attraverso la cartuccia. Sia HybridSPE che [](https://www.sigmaaldrich.com)[QuEChERS](https://www.sigmaaldrich.com/CH/it/applications/analytical-chemistry/sample-preparation/quechers) SPE funzionano attraverso la rimozione delle interferenze. Il metodo SPE ottimale dipende dalla struttura, dalla solubilità, dalla polarità e dalle proprietà lipofile (coefficienti di distribuzione) dell'analita. [](https://www.sigmaaldrich.com)Sono disponibili guide alla selezione per aiutare a scegliere la fase stazionaria e il solvente più favorevoli per l'applicazione di interesse. ## Applicazioni comuni della SPE La SPE è frequentemente utilizzata nei settori farmaceutico, clinico e dei test diagnostici high-throughput, forense, ambientale e alimentare/agrochimico per analisi relative a: - Composti farmaceutici e metaboliti nei liquidi biologici - Farmaci d'abuso nei liquidi biologici - Inquinanti ambientali nelle acque potabili e reflue - Pesticidi, antibiotici o micotossine in matrici alimentari/agricole - Desaltazione di proteine e peptidi - Frazionamento di lipidi - Vitamine idrosolubili e liposolubili [](https://www.sigmaaldrich.com/documents-search) [Cercate informazioni più specifiche?](https://www.sigmaaldrich.com/documents-search) Visita la nostra ricerca di documenti per schede tecniche, certificati e documentazione tecnica. [Trova i documenti](https://www.sigmaaldrich.com/documents-search) ## Articoli correlati - [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. - [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. - [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. - [Analysis of Pesticide Residues in Pistachios Using QuEChERS Extraction and Cleanup with Supel™ QuE Z-Sep+](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-pistachios) Pistachios 45% fat complicates pesticide analysis. But Z-Sep+ sorbent cleanup after QuEChERS extraction can reduce background for reliable results. - [HybridSPE-Phospholipid Technology](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/hybridspe-precipitation-technology) This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed. - [Vedi tutti (41)](https://www.sigmaaldrich.com/CH/it/search/facet-search?focus=sitecontent&term=facet-search) ## Protocolli correlati - [Determinazione del bisfenolo A nelle acque potabili](https://www.sigmaaldrich.com/CH/it/technical-documents/protocol/environmental-testing-and-industrial-hygiene/drinking-water-testing/bisphenol-a-water) Preparazione completa del campione e procedura analitica sviluppate da Supelco per la determinazione del BPA nelle acque potabili. - [Determinazione del triclosano nelle acque ambientali](https://www.sigmaaldrich.com/CH/it/technical-documents/protocol/environmental-testing-and-industrial-hygiene/waste-water-and-process-water-testing/determination-triclosan-water) La presenza del triclosano negli scarichi degli impianti di trattamento delle acque reflue è stata determinata mediante HPLC seguita da rivelazione UV e MS/MS. - [Empowering Biochemists to Analyze Glycoproteins with Exceptional Reproducibility](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-glycan-hplc-columns) Fast, high-resolution separations of procainamide labeled glycans - dextran ladder, human IgG and cetuximab - [Biological Testing for Amphetamine and Methamphetamine Abuse](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/biological-testing-for-amphetamine-and-methamphetamine-abuse) In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine. - [Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/chiral-hplc-amphetamine-methamphetamine) Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine - [Vedi tutti (34)](https://www.sigmaaldrich.com/CH/it/search/facet-search?focus=sitecontent&term=facet-search) ### Trova altri articoli e protocolli Inserire le parole chiave Ricerca ## Come possiamo aiutarvi In caso di domande, inviate una [richiesta di assistenza clienti](https://www.sigmaaldrich.com/CH/it/support/customer-support) o rivolgetevi al nostro servizio clienti: Email [[email protected]](mailto:[email protected]) o chiama +1 (800) 244-1173 ## Supporto aggiuntivo - [Chromatogram Search](https://www.sigmaaldrich.com/chromatogram-search) Use the Chromatogram Search to identify unknown compounds in your sample. - [Strumenti di calcolo e app](https://www.sigmaaldrich.com/CH/it/support/calculators-and-apps) Strumenti e risorse scientifiche online per la chimica analitica, le life science, la sintesi chimica e la scienza dei materiali. - [Customer Support Request](https://www.sigmaaldrich.com/CH/it/support/customer-support) Assistenza clienti inclusa assistenza per ordini, prodotti, account e problemi tecnici del sito web. - [FAQ](https://maestro.my.site.com/knowledgeportal/s/) Explore our Frequently Asked Questions for answers to commonly asked questions about our products and services. * * * __Articoli in evidenza__ [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. [HybridSPE-Phospholipid Technology](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/hybridspe-precipitation-technology) This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed. Top __Autenticati per continuare__ Per continuare a leggere, autenticati o crea un account. 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