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Merck

77607

Sigma-Aldrich

Phenol

BioUltra, Molecular Biology, TE-saturated, ~73% (T)

Synonym(s):

Hydroxybenzene

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CLP 338,000
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250 ML
CLP 338,000
1 L
CLP 1,120,000

About This Item

Linear Formula:
C6H5OH
CAS Number:
Molecular Weight:
94.11
Beilstein:
969616
EC Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:
NACRES:
NA.25
Grade:
Molecular Biology
Bp:
182 °C (lit.)
Vapor pressure:
0.09 psi ( 55 °C)
0.36 mmHg ( 20 °C)

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CLP 338,000


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grade

Molecular Biology

Quality Level

vapor density

3.24 (vs air)

vapor pressure

0.09 psi ( 55 °C)
0.36 mmHg ( 20 °C)

product line

BioUltra

Assay

~73% (T)

form

liquid

autoignition temp.

1319 °F

quality

TE-saturated

contains

~0.1% 8-hydroxyquinoline as preservative

expl. lim.

8.6 %

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.48

pH

~7.8(aqueous phase)

bp

182 °C (lit.)

mp

40-42 °C (lit.)

density

1.071 g/mL at 25 °C (lit.)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

Oc1ccccc1

suitability

suitable for molecular biology

storage temp.

2-8°C

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

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This Item
P5566185450242322
Phenol BioUltra, Molecular Biology, TE-saturated, ~73% (T)

77607

Phenol

Phenol BioXtra, ≥99.5% (GC)

P5566

Phenol

Phenol unstabilized, ReagentPlus®, ≥99%

185450

Phenol

Phenol ACS reagent, ≥99.0%

242322

Phenol

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

assay

~73% (T)

assay

≥99.5% (GC)

assay

≥99%

assay

≥99.0%

grade

for molecular biology

grade

-

grade

-

grade

ACS reagent

form

liquid

form

powder

form

crystalline (flakes), crystalline (solid), crystals

form

loose crystals

refractive index

n20/D 1.48

refractive index

-

refractive index

-

refractive index

-

vapor pressure

0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)

vapor pressure

0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)

vapor pressure

0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)

vapor pressure

0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)

General description

Phenol, also known as hydroxybenzene, is an aromatic compound produced both naturally and synthetically.[1] It acts as a disinfectant and is highly toxic. Exposure to phenol through inhalation, cutaneous adsorption, or oral ingestion causes denaturation and precipitation of cellular protein, which in turn leads to poisoning.[2]
saturated with 10 mM TRIS buffer pH 8.0 and 1 mM EDTA

Application

Phenol (TE-saturated) has been used in the phenol-chloroform extraction procedure for plasmid DNA isolation.[3] It has also been used to extract the aqueous phase during DNA linearization and mRNA synthesis.[4]

Caution

according to temperature, a small quantity of an overlaid aqueous phase may form

Other Notes

RNA fingerprinting - preparation of the E. coli tRNA carrier[5]

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Target Organs

Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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