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77618
Phenol – chloroform – isoamyl alcohol mixture
BioUltra, Molecular Biology, 49.5:49.5:1
Phenol – chloroform – isoamyl alcohol mixture
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
phenol:Chloroform:Isoamyl Alcohol
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352401
PubChem Substance ID:
NACRES:
NA.07
Grade:
Molecular Biology
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grade
Molecular Biology
Quality Level
product line
BioUltra
form
liquid
impurities
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
pH
7.7-8.3(H2O-phase, after extraction with H2O, 1:1)
density
1.29 g/mL at 20 °C
absorption
cut-off at 360 nm
storage temp.
2-8°C
SMILES string
ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
InChI
1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
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This Item | 77619 | 77617 | 516726 |
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade Molecular Biology |
| form liquid | form liquid | form liquid | form liquid (Two-layer) |
| density 1.29 g/mL at 20 °C | density 1.12 g/mL at 20 °C | density 1.28 g/mL at 20 °C | density - |
| pH 7.7-8.3(H2O-phase, after extraction with H2O, 1:1) | pH 4-5(H2O-phase, after extraction with H2O, 1:1) | pH 7.2-8.3(H2O-phase, after extraction with H2O, 1:1) | pH - |
| impurities DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected | impurities - |
Application
- Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).
- Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).
- The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).
- The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).
Physical form
mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline
Other Notes
Large- and small-scale phenol extractions[1]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
Target Organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
177.8 °F - closed cup
Flash Point(C)
81 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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