A7419

Avidin–Peroxidase

buffered aqueous solution

• Synonym(s): Avidin–HRP
• MDL number: MFCD00130587
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: avidin from egg white; enzyme from horseradish
• Quality Level: 200
• conjugate: peroxidase conjugate
• form: buffered aqueous solution
• technique(s): indirect ELISA: 1:100,000; western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for ELISA applications[2][3]. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.

Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml. [4]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Affinity purified protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable