C7749 does not have an affinity tag.
C7749
Carbonic Anhydrase II bovine
≥90% (SDS-PAGE), recombinant, expressed in E. coli, ≥5,000 units/mg protein, buffered aqueous solution
Synonym(s):
Bovine carbonic anhydrase, Carbonic anhydrase enzyme
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500 μG
CLP 212,000
5 MG
CLP 458,000
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500 μG
CLP 212,000
5 MG
CLP 458,000
About This Item
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recombinant
expressed in E. coli
Quality Level
Assay
≥90% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥5,000 units/mg protein
mol wt
~29 kDa
shipped in
wet ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | C6624 | C2522 | C3640 |
|---|---|---|---|
| specific activity ≥5,000 units/mg protein | specific activity ≥5,000 units/mg protein | specific activity ≥3,000 W-A units/mg protein | specific activity ≥2,000 W-A units/mg protein |
| assay ≥90% (SDS-PAGE) | assay - | assay - | assay - |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| mol wt ~29 kDa | mol wt large subunit 17-22 kDa, small subunit 10-12 kDa | mol wt 30 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Application
Carbonic Anhydrase II bovine has been used as a control in native gel electrophoresis and X-ray absorption spectroscopy characterization of Thalassiosira weissflogii carbonic anhydrase 1.[1]
Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques [2]. Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome [3][4]. Product C7749, Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.
Biochem/physiol Actions
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications [4]. Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.
Unit Definition
One unit will decrease th pH of a 20 mM tris buffer from pH 8.3 to 6.3 per minute at 0 °C.
Physical form
Supplied as a solution in 20 mM Tris, pH 7.6, with 150 mM NaCl.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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蛋白質（型番：C7749）に付加されているタグ種類と部位を知りたいです。
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