CAT100

Catalase Assay Kit

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

• Synonym(s): Catalase Activity Detection Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 200
• usage: sufficient for ≥100 tests enzymatic
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... CAT(847)

Description

General description

The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.

Application

Sutitable for Colorimetric and UV Assays

Biochem/physiol Actions

Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H₂O₂ from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.

Features and Benefits

• Useful for determining catalase activity - may be used in various tissues and cells
• Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity

Suitability

Suitable for studying catalase activity in various tissues and subcellular organelles.

Principle

This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm

Unit Definition

One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.

Preparation Note

Use ultrapure water in preparation of all solutions.

Kit Components Also Available Separately

• P6782 Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol) 5 mg
• 323381 Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves