F3651

Fluorescein 5(6)-isothiocyanate

≥90% purity (HPLC), powder

• Synonym(s): FITC, Fluorescein isothiocyanate
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 27072-45-3
• Molecular Weight: 389.38
• Beilstein: 1407295
• EC Number: 248-207-7
• MDL number: MFCD00005063
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894855
• NACRES: NA.47

Properties

• Product Name: Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
• Quality Level: 200
• Assay: ≥90% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to orange
• solubility: acetone: 1 mg/mL, clear, light yellow to yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]

Application

Fluorescein 5(6)-isothiocyanate has been used:

• for fluorescence labeling of bacteria[3]
• as a standard for calibration of fluorometer[4]
• for the labeling of exosomes[5]

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3