H3506

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• EC Number: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type I-S
• Quality Level: 200
• form: lyophilized powder
• specific activity: 400-1000 units/mg solid
• mol wt: ~55 kDa (four subunits of 14 kDa each)
• technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• UniProt accession no.: Q5E985; Q8SQG8
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.[1][2][3]

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves