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P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

Molecular Biology

Synonym(s):

phenol chloroform RNA extraction

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100 ML
CLP 47,800
400 ML
CLP 146,000

CLP 47,800

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100 ML
CLP 47,800
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CLP 146,000

About This Item

UNSPSC Code:
12190000
NACRES:
NA.52
grade:
Molecular Biology
application(s):
life science and biopharma

CLP 47,800

Check Cart for Availability

grade

Molecular Biology

Quality Level

200

Assay

≥99.0%

form

liquid

pH

4.3-4.7

application(s)

life science and biopharma

storage temp.

2-8°C

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This Item
P3803P2069P4682
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) Molecular Biology

P1944

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA Molecular Biology

P3803

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA Supplied with Equilibration buffer, Molecular Biology

P2069

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA

Phenol solution Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

P4682

Phenol solution

assay

≥99.0%

assay

≥99.0%

assay

≥99.0%

assay

-

application(s)

life science and biopharma

application(s)

agriculture

application(s)

agriculture

application(s)

-

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

pH

4.3-4.7

pH

6.5-6.9(pH of phenolic phase)

pH

6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

pH

4.1-4.5 (25 °C)

General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.[1]

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.[2][3][4]
  • to isolate RNA from Arabidopsis siliques.[5]
  • to extract proteins and DNA from polysomal and non-polysomal fractions.[6]
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.[7]

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Target Organs

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

175.0 °F - closed cup

Flash Point(C)

79.44 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW7584
MKCV5362
MKCV5361
MKCT1646
MKCT0359

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