P4244

Phosphate buffered saline

liquid, pH 7.2 (25 °C), autoclaved

• Synonym(s): PBS
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• NACRES: NA.25
• MDL number: MFCD00131855

Properties

• Product Name: Phosphate buffered saline, Autoclaved, pH 7.2 (25 °C)
• description: Sodium chloride, 150 mM, and sodium phosphate, 150 mM
• form: liquid
• pH: 7.2 (25 °C)
• application(s): diagnostic assay manufacturing
• storage temp.: 2-8°C
• Quality Level: 200

Description

Application

Phosphate buffered saline has been used:

• for washing bacterial cells after harvesting by centrifugation[1]
• for washing the peritoneal cavity for isolation of peritoneal macrophages[1]
• as a control and to dilute phytohemagglutinin (PHA) to prepare subcutaneous injections and study the immune response in birds.[2][3]

General description

Phosphate buffered saline (PBS) is an isotonic standard buffer solution.[4] PBS imitates the pH, ion concentrations, and osmolarity of the human body. It serves as an ideal buffer for maintaining the osmolarity and physiological pH for biomedical dilutions in cell culture and protein chemistry. PBS is used for standard enzymatic tests with active metal-organic framework (MOF) biocomposites.[5] PBS is considered non-toxic to cells.[1]

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)