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Merck

P7059

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonym(s):

Phosphate buffered saline, PBS

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1 L

CLP 151,000

CLP 151,000

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About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:
MFCD00131855

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Product Name

Phosphate Buffered Saline, 10× PBS for Western blots and IP

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

pH

7.2-7.6(1x solution)

storage temp.

room temp

Quality Level

200

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1 of 4

This Item
806552806544P4739
Phosphate Buffered Saline 10× PBS for Western blots and IP

Sigma-Aldrich

P7059

Phosphate Buffered Saline

Phosphate Buffered Saline pH 7.4, liquid, sterile-filtered, suitable for cell culture

SAFC

806552

Phosphate Buffered Saline

Phosphate Buffered Saline pH 7.2, liquid, sterile-filtered, suitable for cell culture

SAFC

806544

Phosphate Buffered Saline

Phosphate buffered saline with 5% nonfat milk, powder (dry milled), pH 7.3

Sigma-Aldrich

P4739

Phosphate buffered saline

grade

for molecular biology

grade

-

grade

-

grade

-

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

100

form

liquid

form

liquid

form

liquid

form

powder (dry milled)

pH

7.2-7.6(1x solution)

pH

7.4

pH

7.2

pH

7.3

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Preparation Note

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate
Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000519412
0000519412
0000492632
0000492632
0000468486

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Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
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Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Shahani Noor et al.
Brain, behavior, and immunity, 61, 80-95 (2016-12-25)
A growing body of evidence indicates that prenatal alcohol exposure (PAE) may predispose individuals to secondary medical disabilities later in life. Animal models of PAE reveal neuroimmune sequelae such as elevated brain astrocyte and microglial activation with corresponding region-specific changes
Katie Burbridge et al.
Development (Cambridge, England), 148(8) (2021-04-30)
Body tissues are frequently exposed to stress, from toxic byproducts generated during cellular metabolism through to infection or wounding. Although it is well-established that tissues respond to exogenous injury by rapidly upregulating cytoprotective machinery, how energetically demanding tissues - vulnerable
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