Small molecule HPLC

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Showing 1-30 of 173 results

UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC

Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.

HPLC Tips & Tricks: Mobile Phase Preparation Part 2 - Buffers

Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.

Determination of Tramadol in Urine using LC-MS/MS

This article presents an LC-MS/MS method for the determination of tramadol in urine samples using reversed-phase LC-MS/MS aided by isotope internal standards.

Impact of Mobile Phase Additives on LC-MS Sensitivity, Demonstrated using Spice Cannabinoids

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.

Developing HPLC Methods

We recommend every HPLC method developer have these four columns in their arsenal; Ascentis C18, Ascentis RP-Amide, Ascentis Phenyl and Discovery HS F5.

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Ascentis® Express Biphenyl UHPLC and HPLC Columns

The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the

SupraSolv® GC Solvent Specifications

Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.

Chromatographic Selectivity

Harnessing the Power of Chromatographic Selectivity

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

HPLC Tips & Tricks: Optimizing Injection Volume

Learn the tips and tricks to optimize the injection volume during your High-Performance Liquid Chromatography (HPLC) method-based analyses and further, how it affects your peak area.

Efficient Analysis of Kava Extract: New phytochemical certified reference material mixes for ginger and kava

Learn more about our Kava CRM solution for use in quantitative and qualitative analysis of kavalactones and flavokavains in kava products to confirm their potency and authenticity.

Extractables Studies of Single-Use Equipment Immediate Identification and Quantification of Unknown Extractables by GC/MS with a Certified Reference Material Mix for Extractables and Leachables

Identification and quantification of the most common extractables originating from single-use equipment by GC/MS and an Extractables and Leachables Screening Standard.

USP HPLC Analysis of L-Methionine on Ascentis® Express OH5

Separation of L-Methionine, Pharmaceutical Secondary Standard; Certified Reference Material

Piroxicam Capsules-Assay and Organic Impurities Following United States Pharmacopoeia Pending Forum Method

A reversed-phase high-performance liquid chromatography (RP- HPLC) based quantitative assay and organic impurity analysis of Piroxicam in its capsule dosage form.

Supelco Preparative HPLC products for Pharmaceutical Development and Production

HPLC Tips & Tricks: Getting Greener in HPLC

Discover sustainable liquid chromatography techniques, including eco-friendly solvents and optimized methods, to reduce environmental impact in various analytical fields.

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Sample Cleanliness Using BioSPME

Learn about Supel™ BioSPME C18 96-pin tool to prepare a clean sample with minimal matrix effects for an LC-MS/MS analysis from human plasma and its comparison with acetonitrile-based precipitation.

Famciclovir Tablets USP Monograph Method Using a Purospher™ STAR RP-8 endcapped HPLC Column and UV Detection

Following the new USP monograph for Famciclovir Tablets, Purospher™ STAR RP-8 endcapped HPLC columns can be used to monitor organic impurities in Famciclovir tablet formulations. Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus

Tips & Tricks for IC: A Quick Overview on IQ/ OQ/PV(PQ) of Ion Chromatography Instruments

Explore the tests integral to the instrument qualification process for ion chromatography instruments. Learn about the effectiveness of a pre-packaged IQ/OQ/PQ kit, featuring six certified bromide standard solutions for comprehensive IC instrument qualification tests.

Natural Cannabinoid and Cannflavin Profiling by HPLC-PDA

An accurate and precise HPLC-PDA measurement of 32 cannabinoids and 2 cannflavins in hemp samples using a single extraction in less than 32 minutes.

Application Note – Peroxidase in Vegetables

Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.

Analysis of Caffeine and Catechins in Green Tea

Validated HPLC method (based on Ph. Eur. Monograph 2668) for quantifying catechins and caffeine in decaffeinated green tea using a Chromolith® High Resolution RP-18e column with advantages in terms of backpressure and matrix robustness.

Speed, Simplicity and Accuracy of Protein Binding Determination using BioSPME

Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.

Fluticasone Propionate Assay by HPLC UV USP Monograph

Learn how the Purospher® STAR RP-18e Hibar® HPLC column meets the system suitability criteria outlined in the USP monograph for the fluticasone propionate assay.

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