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Phyllosilicate Clay Minerals: Principles and Applications

We have discussed shortly the applications of 1:1 and 2:1 phyllosilicate material in membrane technology, as well as the basic geometric understanding of their layered structures.

BRAND® Insert 2in1 Supports the Cultivation of Reconstructed Human Epidermis (RhE) used for Skin Corrosion Tests (OECD TG 431)

The BRAND® Insert 2in1 cell culture insert supports the cultivation of Reconstructed Human Epidermis used for skin corrosion tests.

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Cell Freezing and Banking

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.

McCoy's 5A Modified Media Formulation

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Williams' Medium E Formulation

Degrader Building Blocks with Inhibitor of Apoptosis Protein (IAP) In Silico-Derived Ligands

Targeted protein degradation (TPD) is an emerging strategy that uses small molecules to hijack endogenous proteolysis systems to degrade disease-relevant proteins and thus reduce their abundance in cells.

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

Click Chemistry in Drug Discovery

Drug discovery process by utilizing chemistry reaction of Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition of terminal alkynes with organoazides to yield 1,4-disubstituted 1,2,3-triazoles.

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

L-15 Media Formulation

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Retinoic Acid and Gene Expression

All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.

Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models

Sophisticated models are developing as 3D cell culture technology becomes established and accepted as a means of creating more physiologically relevant cell-based assays.

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

High-Throughput Drug Screening using TrueGel3D® HTS Hydrogel Plates

Compound cytotoxicity screening assays with 3D cell cultures using TrueGel3D® HTS Hydrogel Plates including dose response curves, IC50 determination, and spheroid killing assay data.

Tips and Tricks: Millicell^® DCI Digital Cell Imager Cloud Application

Tips and tricks for using the Millicell® Cloud Application to analyze data from the Millicell® DCI digital cell imager.

Modified Amino Acids

Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes.

Introduction to Microbial Media

An introduction to microbial media types, uses and applications. Includes formulations and product selection guide.

Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.

Nanomaterials for Energy Storage in Lithium-ion Battery Applications

Self-Assembly in Organic Thin Film Transistors for Flexible Electronic Devices

Flexible electronic circuits and displays based on organic active materials are future generations of products that may eventually enter mainstream electronics market.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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