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World Science Day Drawing
NO PURCHASE NECESSARY TO ENTER OR WIN. A PURCHASE OR PAYMENT OF ANY KIND WILL NOT INCREASE YOUR CHANCES OF WINNING. NOT OPEN TO LEGAL RESIDENTS OF POLAND, ITALY, SAUDI ARABIA, BURUNDI, CENTRAL AFRICAN REPUBLIC, DEMOCRATIC REPUBLIC OF
Simplifying CRISPR Licensing
Merck has announced a new licensing collaboration with the Broad Institute that can simplify your path to licensing CRISPR technology, while making it more widely available to the global research and discovery community at large.
Phyllosilicate Clay Minerals: Principles and Applications
We have discussed shortly the applications of 1:1 and 2:1 phyllosilicate material in membrane technology, as well as the basic geometric understanding of their layered structures.
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.
Affinity Purification with Ultrafree®-MC Centrifugal Filters
Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step
Lentiviral Titer via p24 (viral coat protein) ELISA Assay
Calculate your lentiviral titer with the p24 ELISA assay. Detailed protcol below.
Pull-down assays
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Performing a Separation of Biotin and Biotinylated Substances with HiTrap Streptavidin HP and Streptavidin Sepharose High Performance
How to separate biotin and biotinylated substances by affinity chromatography using HiTrap Streptavidin HP and Streptavidin Sepharose High Performance products from Cytiva.
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
Enzymatic Assay of Esterase
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Enzymatic Assay of Aprotinin
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Enzymatic Assay of Trypsin Inhibitor
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Sepharose® High Performance: Purification with High Resolution
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins
This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.
Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.
Enzymatic Assay of Mutanolysin
This procedure may be used for Mutanolysin products.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Anti-HA Affinity Matrix Protocol & Troubleshooting
To concentrate the protein eluate, Roche recommends following the immunoprecipitation protocol given in the package insert from step 2 to 5. Use 50 μl of resuspended Anti-HA Affinity Matrix in a microcentrifuge tube.
Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin
This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
Packing Tricorn 10/100 Columns with MabSelect™ or MabSelect™ SuRe
This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from Cytiva.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.
cOmplete™ Protocol
It is possible dissolve 1 tablet in as little as 2 ml double distilled Water, results in a 25x stock solution, without difficulty.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Fractional Precipitation Protocol for Affinity Chromatography Samples
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
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