This antibody is an IgG isotype. The whole protein was used for immunization and the epitope has not been mapped. This is a polyclonal antibody and can bind to more than one region.
AB144P
Anti-Choline Acetyltransferase Antibody
CHEMICON®, goat polyclonal
Synonyma:
Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Vybrat velikost
200 μL
14 000,00 Kč
500 μL
19 400,00 Kč
1 ML
26 300,00 Kč
14 000,00 Kč
Odhaduje se, že bude odesláno dnespodrobné informace
K vašemu cílovému použití je dostupná rekombinantní protilátka bez obsahu konzervačních látek. Vyzkoušejte ZRB1012
Zakoupit v balíčku
O této položce
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Dovolte nám, abychom vám pomohliNázev produktu
Anti-Choline Acetyltransferase Antibody, Chemicon®, from goat
biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)
1 of 4
Tato položka | AB143 | SAB2500236 | MAB305 |
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form serum | antibody form affinity isolated antibody | antibody form ascites fluid |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source goat | biological source rabbit | biological source goat | biological source mouse |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| technique(s) immunocytochemistry: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) indirect ELISA: suitable, western blot: suitable | technique(s) immunohistochemistry: suitable |
General description
70/74 kDa
Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.
Immunogen
Human placental enzyme.
Application
Anti-Choline Acetyltransferase Antibody has been used in:
Immunohistochemistry:
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.
- immunohistochemical staining
- western blotting
- immunocytochemistry
Immunohistochemistry:
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.
Biochem/physiol Actions
Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.
Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
Physical form
Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.
For short term storage (3-4 weeks) maintain at 2-8°C.
Analysis Note
Control
Human placenta lysates, rat forebrain/rostral hypothalamus.
Human placenta lysates, rat forebrain/rostral hypothalamus.
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
Other Notes
Research Category:
Neuroscience
Research Sub Category:
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neuroscience
Research Sub Category:
Neurotransmitters & Receptors
Neuronal & Glial Markers
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Skladovací třída
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Plasticity of motor network and function in the absence of corticospinal projection
Han Q, et al.
Experimental Neurology, 194-208 (2015)
The human choline acetyltransferase gene encodes two proteins
Grosman DD, et al.
Journal of Neurochemistry (1995)
Sigma-1 Receptor Plays a Negative Modulation on N-type Calcium Channel
Zhang K, et al.
Frontiers in Pharmacology, 302-302 (2017)
S D Motts et al.
Neuroscience, 160(1), 103-114 (2009-03-14)
We combined retrograde tracing with immunohistochemistry for choline acetyltransferase to identify the source of cholinergic input to the inferior colliculus (IC) in guinea pigs. Injection of a retrograde tracer into one IC labeled cells in many brainstem nuclei. Retrogradely-labeled cells
Choline acetyltransferase structure reveals distribution of mutations that cause motor disorders
Cai Y, et al.
The Embo Journal, 2047-2047 (2004)
Sortimentní položky
Protilátky se spojují se specifickými antigeny a vytvářejí exkluzivní komplex protilátka-antigen. Seznamte se s podstatou této vazby a jejím využitím jako molekulární značky pro výzkum.
Zjistěte, jaké jsou rozdíly mezi monoklonálními a polyklonálními protilátkami, včetně způsobu generování protilátek, počtu klonů a formátů protilátek.
Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.
Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.
Související obsah
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
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