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Merck

1.25195

SAFC

Cellvento® 4HEK Powder

Chemically defined, powder, suitable (for HEK293 cell culture)

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5 L
CZK 9,430.00
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CZK 15,800.00
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CZK 34,500.00
50 L
CZK 65,900.00
100 L
CZK 128,800.00

CZK 9,430.00


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About This Item

UNSPSC Code:
41106213

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Quality Level

description

shipped with cooling pack.

Dry powder medium must be supplemented with 2.5 g/L Sodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mM L-Glutamine

product line

Cellvento®

form

dry powder

technique(s)

cell culture | mammalian: suitable

suitability

suitable (for HEK293 cell culture)

storage temp.

2-8°C

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This Item
1.2519314390C1.03795
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

form

dry powder

form

liquid

form

liquid

form

dry powder

product line

Cellvento®

product line

Cellvento®

product line

Cellvento®

product line

Cellvento®

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

description

shipped with cooling pack

description

shipped with cooling pack

description

-

description

shipped with cooling packs

suitability

suitable (for HEK293 cell culture)

suitability

-

suitability

-

suitability

-

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Dry powder medium must be supplemented with 2.5 g/LSodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mML-Glutamine.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.

Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.

Upstream gene therapy optimization maximizes viral vector titers; partner with technology experts for HEK293, HEK293T, and Sf9 cell solutions.

Minimize the complexities of cell and gene therapy production. Switch to upstream viral vector platforms to enhance scalability and simplify GMP manufacturing.

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Řešení výroby virových vektorů založené na transfekci s využitím linie suspenzních buněk, chemicky definovaného média a procesu s ověřenou výkonností v měřítku.

A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.

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