480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

• Synonym(s): α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase
• CAS Number: 9001-67-6
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• product line: EMPROVE^® EXPERT
• form: liquid
• specific activity: ≥10 units/mL; ≥250 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Packaging

500 miu in Plastic ampoule

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Warning
• Hazard Statements: H290 - H315 - H319 - H335
• Precautionary Statements: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable