The human panel HNABTMAG-68k follows the Tau standard with isoform Tau441. Both the capture and detection immunogens used are also Tau441. Cross reactivity for other isoforms has not been tested.
HNABTMAG-68K
MILLIPLEX® Human Neuroscience Panel
Configurable Human Amyloid Beta and Tau 4-Plex Panel, allows quantitative multiplex detection of multiple analytes simultaneously
Synonym(s):
Human Neuroscience Protein Multiplex Assay, Luminex® Human Neuroscience Panel, Millipore Human Neuroscience Immunoassay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
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Product Name
MILLIPLEX® Human Neuroscience Panel, Configurable Human Amyloid Beta and Tau 4-Plex Panel, allows quantitative multiplex detection of multiple analytes simultaneously
Quality Level
species reactivity
human
packaging
pkg of 1 ea
manufacturer/tradename
Milliplex®
assay range
accuracy: 109%
(Spike recovery for Aβ1-42)
accuracy: 91%
(Spike recovery for pTau181)
accuracy: 98%
(Spike recovery for tTau)
sensitivity: 1.5 pg/mL
(pTau181; MinDC+2SD)
sensitivity: 10.2 pg/mL
(Aβ1-40; MinDC+2SD)
sensitivity: 14.2 pg/mL
(tTau; MinDC+2SD)
sensitivity: 2.5 pg/mL
(Aβ1-42; MinDC+2SD)
standard curve range: 0.7-500 pg/mL
(pTau181)
standard curve range: 11-8000 pg/mL
(tTau)
standard curve range: 2-2000 pg/mL
(Aβ1-42)
standard curve range: 21-15000 pg/mL
(Aβ1-40)
inter-assay cv: <15%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
input
cerebrospinal fluid(s) (CSF)
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
1 of 4
This Item | HNPMAG-35K | HNDG3MAG-36K | APOMAG-62K |
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| assay range accuracy: 109% | assay range accuracy: 98-118% | assay range accuracy: 108% | assay range accuracy: 85% |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 4 analytes in human cerebrospinal fluid (CSF).Analytes included: Amyloid beta 1-40 (Aβ40), Amyloid beta 1-42 (Aβ42), Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181).Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Disclaimer
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Features and Benefits
Harness Mutliplexing Efficiency: Simultaneously measure both Aβ40 and Aβ42 in a single assay, accelerating your neurodegenerative disease research while maximizing laboratory throughput.Built-in Reliability: Every kit undergoes rigorous validation to deliver unwavering consistency across batches, giving you the confidence to pursue breakthrough discoveries without questioning your data integrity.Biologically Relevant Results: Our specially formulated serum matrix replicates physiological conditions, ensuring your findings accurately measure the analytes of interest.Complete Research Solution: Eliminate procurement headaches with our comprehensive kit containing all essential components, allowing you to focus on scientific discovery rather than logistical challenges.Accelerate Scientific Impact: Transform precise biomarker measurements into actionable insights that drive forward our understanding of Alzheimer′s disease and related amyloidopathies.
General description
Alzheimer′s disease (AD) is a progressive neurodegenerative disorder and the leading cause of dementia, affecting approximately 36 million people worldwide. The disease is characterized by two key features: extracellular amyloid β (Aβ) plaques formed from peptides like Aβ1-40 and Aβ1-42, and intracellular neurofibrillary tangles composed of hyperphosphorylated Tau protein. These biomarkers—reduced soluble Aβ1-42, increased total Tau, and phosphorylated Tau in cerebrospinal fluid—serve as critical indicators of the pathological processes underlying neurodegenerative conditions.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Susan E Boehnke et al.
Alzheimer's & dementia (Amsterdam, Netherlands), 12(1), e12069-e12069 (2020-07-23)
Neurofilament light (NFL) in cerebrospinal fluid (CSF) is elevated in neurodegenerative disease patients, and may track disease progression and treatment. Macaque monkeys are emerging as important translational models of neurodegeneration, and NFL may be a useful biomarker. To determine the
Emma L Robertson et al.
Alzheimer's & dementia (New York, N. Y.), 8(1), e12289-e12289 (2022-04-14)
Monkeys are becoming important translational models of neurodegenerative disease. To facilitate model development, we measured cerebrospinal fluid (CSF) concentrations of key biomarkers in healthy male and female cynomolgus and rhesus macaques. Amyloid beta (Aβ40, Aβ42), tau (total tau [t-tau], phosphorylated
Mohamed Raâfet Ben Khedher et al.
Alzheimer's & dementia (New York, N. Y.), 7(1), e12124-e12124 (2021-02-05)
In brain, extracellular vesicles (EVs) play an essential role in the neuron-glia interface and ensure the crosstalk between the brain and the periphery. Some studies now link the pathway dysfunction of the EVs to apolipoprotein E gene variant (APOE ε4)
Wencheng Yin et al.
Frontiers in neuroscience, 14, 602642-602642 (2021-01-05)
Alzheimer's disease (AD)-related degenerative decline is associated to the presence of amyloid beta (Aβ) plaque lesions and neuro fibrillary tangles (NFT). However, the precise molecular mechanisms linking Aβ deposition and neurological decline are still unclear. Here we combine genome-wide transcriptional
Jessica L Hoffman et al.
International review of neurobiology, 148, 169-230 (2019-11-18)
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that represents the most common cause of dementia in the United States. Although the link between alcohol use and AD has been studied, preclinical research has potential to elucidate neurobiological mechanisms that
Protocols
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
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Can you provide information on the specificity or cross reactivity of Total Tau in the human HNABTMAG-68K?
1 answer-
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Can the Human Amyoid Beta / Tau (HNABTMAG-68K) Milliplex kit, which currently only lists CSF as a sample type, be used with serum/plasma samples?
1 answer-
The HNABTMAG-68K Milliplex kit currently only lists cerebrospinal fluid (CSF) as a sample type. When serum/plasma samples were tested with this kit, a strong matrix effect was observed. As the kit was not validated for serum/plasma, the appropriate Serum Matrix for use could not be ascertained. Furthermore, the expected trend for Amyloid β40 and Amyloid β42 in serum/plasma was not observed.
For serum/plasma samples, we recommend using the validated ELISA kits for Amyloid β40 and 42 (EZHS and EZH42) as they are the preferred assays. Additionally, we offer a Total Tau ELISA (RAB1085).
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Are the antibodies used in the Human Amyloid Beta/Tau (HNABTMAG-68K) kit also able to recognize rat samples?
1 answer-
The AB40 and AB42 antibodies have not been specifically tested for reactivity to rat. However, in a test involving 4 rat serum samples, AB40 was detected in all of the samples, while AB42 was not detectable. Additionally, the capture antibodies for pTau and Total Tau have been found to be reactive to both human and rat, as pTau and Total Tau were detected in 2 out of the 4 rat serum samples tested. It's important to note that the panel has not been tested with rat standards to determine the extent of cross-reactivity.
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What is the recommended concentration of guanidine for Amyloid Beta Immunoassays?
1 answer-
The recommended guanidine concentration for Amyloid Beta Immunoassays should be less than 0.5M in the final sample concentration.
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Could you provide the plate capacity for an LX200 or FM3D instrument when using one box of Sheath Fluid?
1 answer-
The FLEXMAP 3D and LX200 instruments use approximately 10 mL of sheath per sample run when running a 96-well plate and the xPONENT protocol is set to aspirate 50 µL per sample. Therefore, to acquire one 96-well plate, the instruments will use approximately 1L of sheath fluid.
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Is it possible to add other analytes to the Human Amyloid Beta / Tau Panel (HNABTMAG-68K) as SPR kits?
1 answer-
Additional analytes cannot be added to the AB/Tau panel due to differences in the assay procedure. The detection antibody is added at the primary incubation, whereas the other panels have the detection added after the primary incubation.
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How should the plate for the complete Milliplex assay in Sheath Fluid be stored if it can't be read for a couple of hours?
1 answer-
The plate can be stored at 4°C without shaking for up to 24 hours. Before reading, the plate should be shaken at room temperature for at least 20 minutes to bring it back to ambient temperature.
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